A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells

被引：39

作者：

Gen, Yasuyuki ^{[1
]}

Yasui, Kohichiroh ^{[1
]}

Zen, Keika ^{[1
]}

Nakajima, Tomoaki ^{[1
]}

Tsuji, Kazuhiro ^{[1
]}

Endo, Mio ^{[1
]}

Mitsuyoshi, Hironori ^{[1
]}

Minami, Masahito ^{[1
]}

Itoh, Yoshito ^{[1
]}

Tanaka, Shinji ^{[2
]}

Taniwaki, Masafumi ^{[1
]}

Arii, Shigeki ^{[2
]}

Okanoue, Takeshi ^{[1
,3
]}

Yoshikawa, Toshikazu ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Kamigyo Ku, Kyoto 6028566, Japan

[2] Tokyo Med & Dent Univ, Dept HepatobiliaryPancreat Surg, Bunkyo Ku, Tokyo 1138510, Japan

[3] Saiseikai Suita Hosp, Dept Hepatol, Osaka 5640013, Japan

来源：

CANCER LETTERS | 2009年 / 275卷 / 01期

关键词：

ARHGAP5; p190-B RhoGAP; RhoA; Amplification; Hepatocellular carcinoma; COMPARATIVE GENOMIC HYBRIDIZATION; GTP-BINDING PROTEIN; CHROMOSOMAL-ABERRATIONS; MEMBRANE PROTRUSION; BREAST-CANCER; ADHESION; POLARITY; GTPASES; CYTOSKELETON; ETIOLOGY;

D O I：

10.1016/j.canlet.2008.09.036

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

RhoA, a member Of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：27 / 34

页数：8

共 23 条

[1] RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity [J].

Arthur, WT ;

Burridge, K .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (09) :2711-2720

[2] Integrin engagement suppresses RhoA activity via a c-Src-dependent mechanism [J].

Arthur, WT ;

Petch, LA ;

Burridge, K .

CURRENT BIOLOGY, 2000, 10 (12) :719-722

[3] p190-B, a new member of the Rho GAP family, and Rho are induced to cluster after integrin cross-linking [J].

Burbelo, PD ;

Miyamoto, S ;

Utani, A ;

Brill, S ;

Yamada, KM ;

Hall, A ;

Yamada, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (52) :30919-30926

[4]

Chakravarty G, 2000, CELL GROWTH DIFFER, V11, P343

[5]

Chen YJ, 1999, GENE CHROMOSOME CANC, V25, P169, DOI 10.1002/(SICI)1098-2264(199906)25:2<169::AID-GCC13>3.0.CO

[6]

2-I

[7] Positional cloning of ZNF217 and NABC1:: Genes amplified at 20q13.2 and overexpressed in breast carcinoma [J].

Collins, C ;

Rommens, JM ;

Kowbel, D ;

Godfrey, T ;

Tanner, M ;

Hwang, S ;

Polikoff, D ;

Nonet, G ;

Cochran, J ;

Myambo, K ;

Jay, KE ;

Froula, J ;

Cloutier, T ;

Kuo, WL ;

Yaswen, P ;

Dairkee, S ;

Giovanola, J ;

Hutchinson, GB ;

Isola, J ;

Kallioniemi, OP ;

Palazzolo, M ;

Martin, C ;

Ericsson, C ;

Pinkel, D ;

Albertson, D ;

Li, WB ;

Gray, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (15) :8703-8708

[8] Integrin-mediated adhesion regulates cell polarity and membrane protrusion through the Rho family of GTPases [J].

Cox, EA ;

Sastry, SK ;

Huttenlocher, A .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (02) :265-277

[9] Rho GTPases and the actin cytoskeleton [J].

Hall, A .

SCIENCE, 1998, 279 (5350) :509-514

[10] CREB3L4, INTS3, and SNAPAP are targets for the 1q21 amplicon frequently detected in hepatocellular carcinoma [J].

Inagaki, Yoshikazu ;

Yasui, Kohichiroh ;

Endo, Mio ;

Nakajima, Tomoaki ;

Zen, Keika ;

Tsuji, Kazuhiro ;

Minami, Masahito ;

Tanaka, Shinji ;

Taniwaki, Masafumi ;

Itoh, Yoshito ;

Arii, Shigeki ;

Okanoue, Takeshi .

CANCER GENETICS AND CYTOGENETICS, 2008, 180 (01) :30-36

← 1 2 3 →