Sequential binding of import ligands to distinct nucleopore regions during their nuclear import

被引:116
作者
Pante, N
Aebi, U
机构
[1] M. E. Müller Inst. for Microsc., Biozentrum, University of Basel, CH-4056 Basel
关键词
D O I
10.1126/science.273.5282.1729
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Protein import into nuclei is mediated by the nuclear pore complex (NPC) and by cellular factors. To structurally characterize this process, nuclear import of gold-labeled nucleoplasmin was followed by electron microscopy to identify NPC components interacting with the import ligand complex in vivo. Before translocation into the nucleus, nucleoplasmin sequentially bound to two distinct regions. first to the distal part of the cytoplasmic filaments and then at the cytoplasmic entry to the central gated channel, Evidence that the delivery of the import ligand from the first to the second binding region occurred by bending of the cytoplasmic filaments is presented here.
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收藏
页码:1729 / 1732
页数:4
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