Obligatory activator-polymerase addition order at promoters

被引：5

作者：

Zhang, X ^{[1
]}

Schleif, R ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 13期

关键词：

D O I：

10.1093/nar/24.13.2575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of open complex formation were measured by migration retardation assay and DNase I footprinting at the activator-dependent promoters ara P1, lac P1 and gal P1, In each case, the rate of open complex formation was significantly faster if the activator, AraC for ara and CAP for lac and gal, had been added before RNA polymerase. The results indicate that complexes of transcriptional activators, RNA polymerase and promoter can exist in two states, one which can form open complexes rapidly and one which cannot.

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页码：2575 / 2577

页数：3

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