High-resolution axial and lateral position sensing using two-photon excitation of fluorophores by a continuous-wave Nd alpha YAG laser

被引：53

作者：

Florin, EL

Horber, JKH

Stelzer, EHK

机构：

[1] Cell Biophysics Programme, Europ. Molecular Biology Laboratory, D-69117 Heidelberg

来源：

APPLIED PHYSICS LETTERS | 1996年 / 69卷 / 04期

关键词：

CONFOCAL FLUORESCENCE MICROSCOPY; REGIME; TRAP;

D O I：

10.1063/1.118134

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The change in position of fluorescent beads captured inside the focal volume of optical tweezers is monitored using fluorescence emission induced by two-photon absorption of a continuous-wave Nd:YAG laser (lambda = 1064 nm). The displacement of a bead due to interactions with its environment leads to a fluorescence intensity variation that is used to design a novel spatial sensor. We determine changes in the axial position of a CY3-labeled latex bead with a diameter of 1.03 mu m to a precision better than 10 nm. At an intensity of 600 mW/mu m(2) the two-photon bleaching rate is lower than 50% per 2000 s. (C) 1996 American Institute of Physics.

引用

页码：446 / 448

页数：3