Effect of benzylaminopurine on in vitro and in vivo root development in lentil, Lens culinaris Medik

被引:41
作者
Polanco, MC [1 ]
Ruiz, ML [1 ]
机构
[1] UNIV LEON, FAC BIOL, AREA GENET, E-24071 LEON, SPAIN
关键词
Lens culinaris; benzylaminopurine; in vitro culture; mitotic index; roots;
D O I
10.1007/s002990050345
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of benzylaminopurine (BAP) on the formation of roots from lentil shoots regenerated on media containing BAP was studied. Seedling shoot tips, first nodes and bractlets, and immature seeds cultured on the initiation media containing 2.25 or 0.225 mg/l of BAP regenerated multiple bud shoots. The regenerated shoots formed roots in percentages ranging from 4.6 to 39.9% on a rooting medium (R medium) containing 2 mg/l of indoleacetic acid. Rooting success on R medium depended upon the cytokinin used in the initiation media, its concentration, and the time elapsed during shoot formation on these media prior to transplanting regenerated shoots to R medium. In vivo study of root growth of lentil seedlings demonstrated the strong inhibitory effect of BAP on root growth reflected in a drastic reduction of the mitotic index of the root meristem.
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页码:22 / 26
页数:5
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