Hydrolysis kinetics and radical-scavenging activity of gelatin under simulated gastrointestinal digestion

被引：23

作者：

Wang, Lin ^{[1
]}

Liang, Qiufang ^{[1
]}

Chen, Qiuhong ^{[1
]}

Xu, Junmin ^{[2
]}

Shi, Zhujun ^{[1
]}

Wang, Zhenbin ^{[1
]}

Liu, Yang ^{[3
]}

Ma, Haile ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

[2] Zhenjiang Kehua Aquaculture Dev Co Ltd, Zhenjiang 212134, Peoples R China

[3] Zhenjiang Entry Exit Inspect & Quarantine Burea, Zhenjiang 212008, Peoples R China

来源：

FOOD CHEMISTRY | 2014年 / 163卷

基金：

中国博士后科学基金;

关键词：

Gelatin; Hydrolysis kinetics; Radical-scavenging activity; Simulated gastrointestinal digestion; ANTIOXIDANT ACTIVITY; COLLAGEN PEPTIDE; PURIFICATION; INGESTION; DAMAGE; L; GLYCOSAMINOGLYCANS; IDENTIFICATION; FIBRILS; SKIN;

D O I：

10.1016/j.foodchem.2014.04.083

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The hydrolysis kinetics and radical-scavenging activity of gelatin were investigated under simulated gastrointestinal digestion in this study. In the gastric phase, the degree of gelatin hydrolysis increased from 0.17% to 1.20%, while the DPPH radical-scavenging rate increased from 6.27% to 24.56%. Further digestion in the intestinal phase brought the degree of hydrolysis and radical-scavenging rate to 26.08% and 44.76%, respectively. After digestion, the gelatin hydrolysates were separated into two fractions by ultrafiltration. The fraction with an average molecular weight of 312.98 Da exhibited the higher yield (78.26%) and radical-scavenging activity (IC50 = 2.09 mg/ml), suggesting the high digestibility and bioactivity of gelatin after oral administration. The fraction was further purified with multi-step column chromatography and identified to be Gly-Pro-Met (303.38 Da) by UPLC-ESI-MS. These results may help us to better understand its physiological effects and to use it properly in foods and pharmaceuticals. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：1 / 5

页数：5

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