Stitching together the Multiple Dimensions of Autophagy Using Metabolomics and Transcriptomics Reveals Impacts on Metabolism, Development, and Plant Responses to the Environment in Arabidopsis

被引:124
作者
Masclaux-Daubresse, Celine [1 ,2 ]
Clement, Gilles [1 ,2 ]
Anne, Pauline [1 ,2 ]
Routaboul, Jean-Marc [1 ,2 ]
Guiboileau, Anne [1 ,2 ]
Soulay, Fabienne [1 ,2 ]
Shirasu, Ken [3 ]
Yoshimoto, Kohki [1 ,2 ,3 ]
机构
[1] INRA, Unite Mixte Rech 1318, Inst Jean Pierre Bourgin, F-78026 Versailles, France
[2] AgroParisTech, Inst Jean Pierre Bourgin, F-78026 Versailles, France
[3] RIKEN, Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
基金
日本学术振兴会;
关键词
INNATE IMMUNE-RESPONSE; PROGRAMMED CELL-DEATH; LEAF SENESCENCE; NITROGEN REMOBILIZATION; VACUOLAR INCLUSIONS; SELECTIVE AUTOPHAGY; GENE-EXPRESSION; THALIANA; PROTEIN; BIOSYNTHESIS;
D O I
10.1105/tpc.114.124677
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Autophagy is a fundamental process in the plant life story, playing a key role in immunity, senescence, nutrient recycling, and adaptation to the environment. Transcriptomics and metabolomics of the rosette leaves of Arabidopsis thaliana autophagy mutants (atg) show that autophagy is essential for cell homeostasis and stress responses and that several metabolic pathways are affected. Depletion of hexoses, quercetins, and anthocyanins parallel the overaccumulation of several amino acids and related compounds, such as glutamate, methionine, glutathione, pipecolate, and 2-aminoadipate. Transcriptomic data show that the pathways for glutathione, methionine, raffinose, galacturonate, and anthocyanin are perturbed. Anthocyanin depletion in atg mutants, which was previously reported as a possible defect in flavonoid trafficking to the vacuole, appears due to the downregulation of the master genes encoding the enzymes and regulatory proteins involved in flavonoid biosynthesis. Overexpression of the PRODUCTION OF ANTHOCYANIN PIGMENT1 transcription factor restores anthocyanin accumulation in vacuoles of atg mutants. Transcriptome analyses reveal connections between autophagy and (1) salicylic acid biosynthesis and response, (2) cytokinin perception, (3) oxidative stress and plant defense, and possible interactions between autophagy and the COP9 signalosome machinery. The metabolic and transcriptomic signatures identified for the autophagy mutants are discussed and show consistencies with the observed phenotypes.
引用
收藏
页码:1857 / 1877
页数:21
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