A rapid and improved technique for scanning electron microscopy of actinomycetes

被引：20

作者：

Prakash, Divya ^{[1
]}

Nawani, N. N. ^{[1
]}

机构：

[1] Dr DY Patil Vidyapeeth, Dr DY Patil Biotechnol & Bioinformat Inst, Pune 411033, Maharashtra, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 99卷

关键词：

Actinomycetes; Lyophilization; Scanning electron microscopy;

D O I：

10.1016/j.mimet.2014.02.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for the preparation of actinomycete cultures before scanning electron microscopy (SEM) is reported. Streptomyces strains were grown in starch casein broth with sterile glass wool as support. The cultures were dehydrated by lyophilization eliminating the use of chemical fixatives and dehydrating agents. (C) 2014 Elsevier B.V. All rights reserved.

引用

收藏

页码：54 / 57

页数：4

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