The development of cryo-EM into a mainstream structural biology technique

被引：299

作者：

Nogales, Eva ^{[1
,2
,3
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Mol Biophys & Integrat Bioimaging Div, Berkeley, CA 94720 USA

来源：

NATURE METHODS | 2016年 / 13卷 / 01期

关键词：

ELECTRON CRYOMICROSCOPY; CRYOELECTRON MICROSCOPY; 3-DIMENSIONAL RECONSTRUCTION; RESOLUTION; COMPLEX; VISUALIZATION; REFINEMENT; CHANNEL; PROTEIN; VIRUS;

D O I：

10.1038/nmeth.3694

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying challenging systems that otherwise defy structural characterization. Recent technical advances have resulted in a 'quantum leap' in applicability, throughput and achievable resolution that has gained this technique worldwide attention. Here I discuss some of the major historical landmarks in the development of the cryo-EM field, ultimately leading to its present success.

引用

收藏

页码：24 / 27

页数：4

相关论文

共 40 条

[1] An atomic structure of human γ-secretase [J].

Bai, Xiao-chen ;

Yan, Chuangye ;

Yang, Guanghui ;

Lu, Peilong ;

Ma, Dan ;

Sun, Linfeng ;

Zhou, Rui ;

Scheres, Sjors H. W. ;

Shi, Yigong .

NATURE, 2015, 525 (7568) :212-+

[2] Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles [J].

Bai, Xiao-chen ;

Fernandez, Israel S. ;

McMullan, Greg ;

Scheres, Sjors H. W. .

[3] 2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor [J].

Bartesaghi, Alberto ;

Merk, Alan ;

Banerjee, Soojay ;

Matthies, Doreen ;

Wu, Xiongwu ;

Milne, Jacqueline L. S. ;

Subramaniam, Sriram .

SCIENCE, 2015, 348 (6239) :1147-1151

[4] Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].

Bottcher, B ;

Wynne, SA ;

Crowther, RA .

NATURE, 1997, 386 (6620) :88-91

[5] Beam-induced motion of vitrified specimen on holey carbon film [J].

Brilot, Axel F. ;

Chen, James Z. ;

Cheng, Anchi ;

Pan, Junhua ;

Harrison, Stephen C. ;

Potter, Clinton S. ;

Carragher, Bridget ;

Henderson, Richard ;

Grigorieff, Nikolaus .

JOURNAL OF STRUCTURAL BIOLOGY, 2012, 177 (03) :630-637

[6] 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy [J].

Campbell, Melody G. ;

Veesler, David ;

Cheng, Anchi ;

Potter, Clinton S. ;

Carragher, Bridget .

ELIFE, 2015, 4 :1-22

[7] Human TFIID Binds to Core Promoter DNA in a Reorganized Structural State [J].

Cianfrocco, Michael A. ;

Kassavetis, George A. ;

Grob, Patricia ;

Fang, Jie ;

Juven-Gershon, Tamar ;

Kadonaga, James T. ;

Nogales, Eva .

CELL, 2013, 152 (1-2) :120-131

[8] Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy [J].

Conway, JF ;

Cheng, N ;

Zlotnick, A ;

Wingfield, PT ;

Stahl, SJ ;

Steven, AC .

NATURE, 1997, 386 (6620) :91-94

[9] PROCEDURES FOR 3-DIMENSIONAL RECONSTRUCTION OF SPHERICAL VIRUSES BY FOURIER SYNTHESIS FROM ELECTRON MICROGRAPHS [J].

CROWTHER, RA .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1971, 261 (837) :221-+

[10] RECONSTRUCTION OF 3 DIMENSIONAL STRUCTURES FROM ELECTRON MICROGRAPHS [J].

DEROSIER, DJ ;

KLUG, A .

NATURE, 1968, 217 (5124) :130-&

← 1 2 3 4 →