A simple and effective coumarin-based fluorescent probe for cysteine

被引：129
作者：
Dai, Xi [1 ]
Wu, Qing-Hua [2 ]
Wang, Peng-Chong [2 ]
Tian, Jie [1 ]
Xu, Yu [1 ]
Wang, Sheng-Qing [1 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
机构：
[1] Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China
[2] Shandong Univ, Inst Dev Biol, Sch Life Sci, Jinan 250100, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2014年 / 59卷
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Cysteine; Coumarin; Conjugate addition; Calf serum; Cell imaging; SELECTIVE DETECTION; BIOLOGICAL THIOLS; LIVING CELLS; BIOIMAGING APPLICATIONS; REDUCED GLUTATHIONE; RATIONAL DESIGN; DUAL-EMISSION; HOMOCYSTEINE; SENSOR; NANOCLUSTERS;
D O I：
10.1016/j.bios.2014.03.018
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657 mu M and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra. (C) 2014 Elsevier BM. All rights reserved.
引用
收藏
页码：35 / 39
页数：5
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