Ehrlich and Sarcoma 180 Tumour Characterisation and Early Detection by 1H NMR-Based Metabonomics of Mice Serum

被引：1

作者：

Grandizoli, Caroline W. P. da S. ^{[1
]}

Carrenho, Luise Z. B. ^{[2
]}

de Francisco, Thais M. G. ^{[2
]}

de Santana Filho, Arquimedes P. ^{[3
]}

Sassaki, Guilherme L. ^{[3
]}

Simonelli, Fabio ^{[1
]}

Nagata, Noemi ^{[1
]}

Kreuger, Maria R. O. ^{[4
]}

Campos, Francinete R. ^{[2
]}

Barison, Andersson ^{[1
]}

机构：

[1] Parana Fed Univ UFPR, NMR Ctr, Dept Chem, BR-81530110 Curitiba, Parana, Brazil

[2] Parana Fed Univ UFPR, Dept Pharm, BR-80210170 Curitiba, Parana, Brazil

[3] Parana Fed Univ UFPR, NMR Ctr, Dept Biochem, BR-81530110 Curitiba, Parana, Brazil

[4] Itajai Valley Univ UNIVALI, Hlth Sci Ctr, BR-88302202 Itajai, SC, Brazil

来源：

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2014年 / 25卷 / 05期

关键词：

cancer; biomarker; NMR; metabonomics; chemometrics; PATTERN-RECOGNITION; BLOOD-SERUM; CANCER; URINE; SPECTROSCOPY; METABOLOMICS; LIPIDS; CLASSIFICATION; EXTRACTION; SYSTEMS;

D O I：

10.5935/0103-5053.20140045

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The success of cancer treatment is directly related to early detection before symptoms emerge, although nowadays few cancers can be detected early. In this sense, 1H nuclear magnetic resonance (1H NMR)-based metabonomics was used to identify metabolic changes in biofluid as a consequence of tumours growing in mice. Through partial least squares discriminant analysis (PLS-DA) analysis of 1H NMR spectra from serum samples it was possible to diagnose Ehrlich ascites and Sarcoma 180 tumours five and ten days after cell inoculation, respectively. Lipids, lipoproteins and lactate were the main biomarkers at onset as well as in the progress of carcinogenic process. Thus, NMR-based metabonomics can be a valuable tool to study the effects of tumour establishment on the chemical composition of biofluids.

引用

页码：841 / 852

页数：12

共 43 条

[1] 1H NMR-based metabonomic analysis of urine from young spontaneously hypertensive rats [J].

Akira, Kazuki ;

Masu, Shigenori ;

Imachi, Misako ;

Mitome, Hidemichi ;

Hashimoto, Miho ;

Hashimoto, Takao .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2008, 46 (03) :550-556

[2]

[Anonymous], 1999, Introduction to multi-and megavariate data analysis using projection methods (PCA & PLS)

[3] High-throughput 1H NMR-based metabolic analysis of human serum and urine for large-scale epidemiological studies:: validation study [J].

Barton, Richard H. ;

Nicholson, Jeremy K. ;

Elliott, Paul ;

Holmes, Elaine .

INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 2008, 37 :31-40

[4]

Bathen TF, 2000, ANTICANCER RES, V20, P2393

[5] Ketones and lactate "fuel" tumor growth and metastasis Evidence that epithelial cancer cells use oxidative mitochondrial metabolism [J].

Bonuccelli, Gloria ;

Tsirigos, Aristotelis ;

Whitaker-Menezes, Diana ;

Pavlides, Stephanos ;

Pestell, Richard G. ;

Chiavarina, Barbara ;

Frank, Philippe G. ;

Flomenberg, Neal ;

Howell, Anthony ;

Martinez-Outschoorn, Ubaldo E. ;

Sotgia, Federica ;

Lisanti, Michael P. .

CELL CYCLE, 2010, 9 (17) :3506-3514

[6] Differential effects of delivery of omega-3 fatty acids to human cancer cells by low-density lipoproteins versus albumin [J].

Edwards, IJ ;

Berquin, IM ;

Sun, HG ;

O'Flaherty, JT ;

Daniel, LW ;

Thomas, MJ ;

Rudel, LL ;

Wykle, RL ;

Chen, YQ .

CLINICAL CANCER RESEARCH, 2004, 10 (24) :8275-8283

[7] Metabolic fingerprinting as a diagnostic tool [J].

Ellis, David I. ;

Dunn, Warwick B. ;

Griffin, Julian L. ;

Allwood, J. William ;

Goodacre, Royston .

PHARMACOGENOMICS, 2007, 8 (09) :1243-1266

[8] Structure-based profiling of metabolites and isotopomers by NMR [J].

Fan, Teresa W. -M. ;

Lane, Andrew N. .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2008, 52 (2-3) :69-117

[9]

Glunde K., 2006, NAT REV CANCER, V11, P835

[10] Choline metabolism in cancer: implications for diagnosis and therapy [J].

Glunde, Kristine ;

Jacobs, Michael A. ;

Bhujwalla, Zaver M. .

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2006, 6 (06) :821-829

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