Aliikangiella marina gen. nov., sp nov., a marine bacterium from the culture broth of Picochlorum sp 122, and proposal of Kangiellaceae fam. nov in the order Oceanospirillales

A Gram-stain-negative, non-motile, non-spore-forming, long rod-shaped bacterium, designated strain GYP-15(T), was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Phylogenetic analyses revealed that strain GYP-15(T) shared 90.6 % 16S rRNA gene sequence similarity with its closest relative, Kangiella aquimarina KCTC 12183(T), and represents a distinct phylogenetic lineage in a robust Glade consisting of GYP-15(T) and members of the genera Kangiella and Pleionea in the order Oceanospirillales. Chemotaxonomic and physiological characteristics, including major cellular fatty acids, NaCI tolerance-and pattern of carbon source utilization, could also readily distinguish strain GYP-15(T) from all established genera and species. Thus, it is concluded that strain GYP-15(T) represents a novel species of a new genus, for which the name Aliikangiella marina gen. nov., sp. nov. is proposed. The type strain of Aliikangiella marina is GYP-15(T) (=MCCC 1K01163(T)=KCTC 42667(T)). Based on phylogenetic results, 16S rRNA gene signature nucleotide pattern and some physiological characteristics, the three genera Kangiella, Pleionea and Aliikangiella are proposed to make up a novel family, Kangiellaceae fam. nov., in the order Oceanospirillales.