How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry

被引：83

作者：

Alcaide, F. ^{[1
]}

Amlerova, J. ^{[2
]}

Bou, G. ^{[3
]}

Ceyssens, P. J. ^{[4
]}

Coll, P. ^{[5
]}

Corcoran, D. ^{[6
]}

Fangous, M. -S. ^{[7
]}

Gonzalez-Alvarez, I. ^{[8
]}

Gorton, R. ^{[9
]}

Greub, G. ^{[10
,11
]}

Hery-Arnaud, G. ^{[7
]}

Hrabak, J. ^{[2
]}

Ingebretsen, A. ^{[12
]}

Lucey, B. ^{[13
]}

Marekovic, I. ^{[14
]}

Mediavilla-Gradolph, C. ^{[15
]}

Monte, M. R. ^{[16
]}

O'Connor, J. ^{[13
]}

O'Mahony, J. ^{[13
]}

Opota, O. ^{[10
]}

O'Reilly, B. ^{[6
]}

Orth-Hoeller, D. ^{[17
]}

Oviano, M. ^{[3
]}

Palacios, J. J. ^{[8
]}

Palop, B. ^{[15
]}

Pranada, A. B. ^{[18
]}

Quiroga, L. ^{[19
]}

Rodriguez-Temporal, D. ^{[1
]}

Ruiz-Serrano, M. J. ^{[11
,19
,20
]}

Tudo, G. ^{[16
]}

Van den Bossche, A. ^{[4
]}

van Ingen, J. ^{[21
]}

Rodriguez-Sanchez, B. ^{[11
,19
,20
]}

机构：

[1] Hosp Univ Bellvitge IDIBELL, Serv Microbiol, Barcelona, Spain

[2] Fac Med Plzen, Biomed Ctr, Plzen, Czech Republic

[3] Hosp Univ A Coruna, Serv Microbiol, La Coruna, Spain

[4] Sci Inst Publ Hlth, Div Bacterial Dis, Brussels, Belgium

[5] Hosp St Pau i Santa Creu, Serv Microbiol, Barcelona, Spain

[6] Cork Univ Hosp, Lab Med, Diagnost Directorate, Cork, Ireland

[7] Hop Cavale Blanche, Plateforme Biol, Lab Bacteriol, Brest, France

[8] Hosp Univ Cent Asturias, Serv Microbiol, Unidad Referencia Reg Micobacterias, Oviedo, Spain

[9] Hlth Serv Labs, London, England

[10] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland

[11] European Study Grp Genom & Mol Diag ESGMD, Basel, Switzerland

[12] Oslo Univ Hosp, Dept Microbiol, Oslo, Norway

[13] Cork Inst Technol, Dept Biol Sci, Cork, Ireland

[14] Univ Zagreb, Univ Hosp Ctr Zagreb, Sch Med, Dept Clin & Mol Microbiol, Zagreb, Croatia

[15] Hosp Reg Malaga, Lab Microbiol, Malaga, Spain

[16] Univ Barcelona, Hosp Clin Barcelona ISGlobal, Dept Fonaments Clin, Lab Microbiol CDB,Fac Med & Ciencies Salut, Barcelona, Spain

[17] Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria

[18] MVZ Dr Eberhard & Partner Dortmund UBAG, Dept Med Microbiol, Dortmund, Germany

[19] Hosp Gen Univ Gregorio Maranon, Inst Invest Sanitaria Gregorio Maranon, Clin Microbiol & Infect Dis Dept, Madrid, Spain

[20] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid, Spain

[21] Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, Nijmegen, Netherlands

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2018年 / 24卷 / 06期

关键词：

Identification; MALDI-TOF MS; Non-tuberculous mycobacteria; Procedure; Sample preparation; DESORPTION IONIZATION-TIME; HEATER-COOLER UNITS; IDENTIFICATION; MS; CHIMERA; SPP; BIOTYPER; BACTERIA; SYSTEMS;

D O I：

10.1016/j.cmi.2017.11.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application in the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS. Aims: The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases, and the interpretation of the results. Sources: Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods, or the implementation of improved databases are discussed. Content: Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided. (C) 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：599 / 603

页数：5

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