Magnesium sulfate fails to reduce infarct volume following transient focal cerebral ischemia in rats

被引:25
作者
Zhu, DH
Martin, R
Meloni, B
Oltvolgyi, C
Moore, S
Majda, B
Knuckey, N [1 ]
机构
[1] Univ Western Australia, Sir Charles Gairdner Hosp, Australian Neuromuscular Res Inst, Dept Neurosurg,Ctr Neuromuscular & Neurol Disorde, Nedlands, WA 6009, Australia
[2] Australian Natl Univ, Sch Biochem & Mol Biol, Canberra, ACT, Australia
基金
英国医学研究理事会;
关键词
focal cerebral ischemia; MgSO4; intraluminal thread method; middle cerebral artery occlusion;
D O I
10.1016/j.neures.2004.04.001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Studies on the neuroprotective effect of magnesium treatment in animal models of focal and global cerebral ischemia have produced inconsistent results. Nevertheless, two magnesium acute stroke phase III trials (IMAGES and FAST-MAG) have either been completed or are planned. Therefore, we decided to re-evaluate the efficacy of magnesium following focal cerebral ischaemia in rats. Two experiments were carried out in two independent laboratories based in Australia. Both used the intraluminal thread method to induce focal cerebral ischemia in the rat. In the Perth study the middle cerebral artery (MCA) was occluded for 45 min and body temperature was controlled during and after ischemia. In the Canberra laboratory the MCA was occluded for 2 h and body temperature was only controlled during Surgery. Three different doses (180, 360, or 720 mumol/kg) of MgSO4 in the Perth study and two different MgSO4 doses (370 or 740 mumol/kg) in the Canberra study were intravenously or intra-arterially administered immediately before ischemia. Control animals were given an equal volume of normal saline just before ischemia in both studies. Twenty-four or 72 h post-ischemia, infarct volume was determined following 2',3',5'-triphenyl-2H-tetrazolium chloride (TTC) staining. No significant differences (P > 0.05) in total, cortical and striatal infarct volumes between saline and MgSO4 treated animals were observed in either study. We conclude MgSO4 does not reduce infarct volume when administered before focal cerebral ischemia in rats. (C) 2004 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.
引用
收藏
页码:347 / 353
页数:7
相关论文
共 33 条
[1]   RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION [J].
BEDERSON, JB ;
PITTS, LH ;
TSUJI, M ;
NISHIMURA, MC ;
DAVIS, RL ;
BARTKOWSKI, H .
STROKE, 1986, 17 (03) :472-476
[2]   Treatment of comatose survivors of out-of-hospital cardiac arrest with induced hypothermia [J].
Bernard, SA ;
Gray, TW ;
Buist, MD ;
Jones, BM ;
Silvester, W ;
Gutteridge, G ;
Smith, K .
NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (08) :557-563
[3]   EFFECTS OF ELEVATED PLASMA MAGNESIUM VERSUS CALCIUM ON CEREBRAL ISCHEMIC-INJURY IN RATS [J].
BLAIR, JL ;
WARNER, DS ;
TODD, MM .
STROKE, 1989, 20 (04) :507-512
[4]  
BUCHAN A, 1990, J NEUROSCI, V10, P311
[5]   MK-801 REDUCED CEREBRAL ISCHEMIC-INJURY BY INDUCING HYPOTHERMIA [J].
CORBETT, D ;
EVANS, S ;
THOMAS, C ;
WANG, D ;
JONAS, RA .
BRAIN RESEARCH, 1990, 514 (02) :300-304
[6]   Diazepam-induced neuroprotection: dissociating the effects of hypothermia following global ischemia [J].
Dowden, J ;
Reid, C ;
Dooley, P ;
Corbett, D .
BRAIN RESEARCH, 1999, 829 (1-2) :1-6
[7]  
Holzer M, 2002, NEW ENGL J MED, V346, P549
[8]   REDUCTION OF INFARCT VOLUME BY MAGNESIUM AFTER MIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS [J].
IZUMI, Y ;
ROUSSEL, S ;
PINARD, E ;
SEYLAZ, J .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1991, 11 (06) :1025-1030
[9]   Effects of delayed intraischemic and postischemic hypothermia on a focal model of transient cerebral ischemia in rats [J].
Kawai, N ;
Okauchi, M ;
Morisaki, K ;
Nagao, S .
STROKE, 2000, 31 (08) :1982-1989
[10]   Expression of c-fos, heat shock protein 70, neurotrophins, and cyclooxygenase-2 mRNA in response to focal cerebral ischemia/reperfusion in rats and their modification by magnesium sulfate [J].
Kinoshita, Y ;
Ueyama, T ;
Senba, E ;
Terada, T ;
Nakai, K ;
Itakura, T .
JOURNAL OF NEUROTRAUMA, 2001, 18 (04) :435-445