Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement

被引：299

作者：

Raffaele, Sylvain ^{[1
]}

Bayer, Emmanuelle ^{[2
]}

Lafarge, David ^{[1
]}

Cluzet, Stephanie ^{[3
]}

Retana, Sylvie German ^{[4
]}

Boubekeur, Tamy ^{[5
]}

Leborgne-Castel, Nathalie ^{[6
]}

Carde, Jean-Pierre ^{[7
]}

Lherminier, Jeannine ^{[6
]}

Noirot, Elodie ^{[6
]}

Satiat-Jeunemaitre, Beatrice ^{[8
]}

Laroche-Traineau, Jeanny ^{[1
]}

Moreau, Patrick ^{[1
]}

Ott, Thomas ^{[9
]}

Maule, Andrew J. ^{[10
]}

Reymond, Philippe ^{[3
]}

Simon-Plas, Francoise ^{[6
]}

Farmer, Edward E. ^{[3
]}

Bessoule, Jean-Jacques ^{[1
]}

Mongrand, Sebastien ^{[1
]}

机构：

[1] Univ Bordeaux, CNRS, UMR 5200, Lab Biogenese Membranaire, F-33076 Bordeaux, France

[2] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland

[3] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland

[4] Univ Bordeaux 2, INRA, UMR Genom Diversite & Pouvoir Pathogene 1090, F-33883 Villenave Dornon, France

[5] Univ Bordeaux, CNRS, Lab Bordelais Rech Informat, UMR 5800, F-33076 Bordeaux, France

[6] Univ Bourgogne, CNRS, INRA 5184, UMR Plant Microbe Environm 1088, F-21065 Dijon, France

[7] Inst Federatif Rech 103, INRA, F-33883 Villenave Dornon, France

[8] CNRS, Inst Sci Vegetal, Lab Dynam Compartimentat Cellulaire, Unite Propre Rech 2355, F-91198 Gif Sur Yvette, France

[9] UMR 2594 441, Lab Interact Plantes Microorganismes, F-31326 Castanet Tolosan, France

[10] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL | 2009年 / 21卷 / 05期

基金：

瑞士国家科学基金会;

关键词：

DETERGENT-RESISTANT MEMBRANES; PLASMA-MEMBRANE; LIPID RAFTS; H+-ATPASE; PLANT-CELLS; ARABIDOPSIS; GENE; EXPRESSION; PURIFICATION; MICRODOMAINS;

D O I：

10.1105/tpc.108.064279

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in similar to 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels in transgenic tomato (Solanum lycopersicum) plants, we show that Potato virus X (PVX) movement is inversely related to REM accumulation. We show that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX. Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane.

引用

页码：1541 / 1555

页数：15

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