Evaluation of brain SERT with 4-[18F]-ADAM/micro-PET and hearing protective effects of dextromethorphan in hearing loss rat model

被引：2
作者：
Liu, Cheng-Tsung [1 ,2 ]
Huang, Yuahn-Sieh [3 ]
Chen, Hsin-Chien [4 ]
Ma, Kuo-Hsing [3 ]
Wang, Chih-Hung [1 ,5 ]
Chiu, Chuang-Hsin [6 ]
Shih, Jui-Hu [2 ,7 ]
Kang, Hsiao-Hsien [8 ]
Shiue, Chyng-Yann [6 ]
Li, I-Hsun [1 ,2 ,7 ]
机构：
[1] Natl Def Med Ctr, Grad Inst Med Sci, Taipei, Taiwan
[2] Triserv Gen Hosp, Dept Pharm Practice, Taipei, Taiwan
[3] Natl Def Med Ctr, Dept Biol & Anat, Taipei, Taiwan
[4] Triserv Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Taipei, Taiwan
[5] Taichung Armed Forces Gen Hosp, Dept Otorhinolcuyngol, Taichung, Taiwan
[6] Triserv Gen Hosp, Dept Nucl Med, Taipei, Taiwan
[7] Natl Def Med Ctr, Sch Pharm, Taipei, Taiwan
[8] Universal Eye Ctr, Taipei, Taiwan
来源：
TOXICOLOGY AND APPLIED PHARMACOLOGY | 2019年 / 378卷
关键词：
Dextromethorphan; 4-[F-18]-ADAM; SERT; Micro-PET; Noise-Induced Hearing Loss; SEROTONIN TRANSPORTER; PLASTICITY; NEUROTRANSMISSION; DAMAGE;
D O I：
10.1016/j.taap.2019.114604
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
This study investigated the protective effects of dextromethorphan (DXM) on noise-induced hearing loss (NIHL) in rats. This study aimed to improve the auditory threshold and to understand the protective effects of DXM against N-methyl-D-aspartate (NMDA)-induced neurite degeneration of serotonergic neurons. The animals were exposed to 8-kHz narrowband noise at a 118-dB sound pressure level for 3.5 h. The hearing thresholds were determined by measuring the auditory brainstem response to click stimuli. Serotonin transporter (SERT) expression was determined through micro-positron emission tomography (PET) using N,N-dimethyl-2-(2-amino-4-F-18-fluorophenylthio)benzylamine (4-[F-18]-ADAM). We also investigated the effects of DXM on NMDA-induced morphological changes in the primary cultures of rat serotonergic neurons. NIHL significantly improved after prophylactic treatment with DXM (p < .05). SERT density in DXM-treated rats was significantly higher than that in non-DXM-treated rats. Because prophylactic medication restored the NMDA-inhibited neurite length of serotonergic neurons and presented SEAT density, DXM could be a potential agent in alleviating NIHL.
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页数：10
相关论文
共 42 条
  • [11] Intercellular Glutamate Signaling in the Nervous System and Beyond
    Featherstone, David E.
    [J]. ACS CHEMICAL NEUROSCIENCE, 2010, 1 (01): : 4 - 12
  • [12] Differential Impact of Temporary and Permanent Noise-Induced Hearing Loss on Neuronal Cell Density in the Mouse Central Auditory Pathway
    Groeschel, Moritz
    Goetze, Romy
    Ernst, Arne
    Basta, Dietmar
    [J]. JOURNAL OF NEUROTRAUMA, 2010, 27 (08) : 1499 - 1507
  • [13] Spatiotemporal definition of neurite outgrowth, refinement and retraction in the developing mouse cochlea
    Huang, Lin-Chien
    Thorne, Peter R.
    Housley, Gary D.
    Montgomery, Johanna M.
    [J]. DEVELOPMENT, 2007, 134 (16): : 2925 - 2933
  • [14] An improved synthesis of 4-[18F]-ADAM, a potent serotonin transporter imaging agent
    Huang, Ya-Yao
    Huang, Wen-Sheng
    Chu, Tieh-Chi
    Shiue, Chyng-Yann
    [J]. APPLIED RADIATION AND ISOTOPES, 2009, 67 (06) : 1063 - 1067
  • [15] Investigating the effects of noise-induced hearing loss on serotonin transporters in rat brain using 4-[18F]-ADAM/small animal PET
    Kang, Hsiao-Hsien
    Wang, Chih-Hung
    Chen, Hsin-Chien
    Li, I-Hsun
    Cheng, Cheng-Yi
    Liu, Ren-Shyan
    Huang, Wen-Sheng
    Shiue, Chyng-Yann
    Ma, Kuo-Hsing
    [J]. NEUROIMAGE, 2013, 75 : 262 - 269
  • [16] Method for serum-free culture of late fetal and early postnatal rat brainstem neurons
    Kivell, BM
    McDonald, FJ
    Miller, JH
    [J]. BRAIN RESEARCH PROTOCOLS, 2001, 6 (03): : 91 - 99
  • [17] Involvement of autophagy upregulation in 3,4-methylenedioxymethamphetamine ('ecstasy')-induced serotonergic neurotoxicity
    Li, I-Hsun
    Ma, Kuo-Hsing
    Kao, Tzu-Jen
    Lin, Yang-Yi
    Weng, Shao-Ju
    Yen, Ting-Yin
    Chen, Lih-Chi
    Huang, Yuahn-Sieh
    [J]. NEUROTOXICOLOGY, 2016, 52 : 114 - 126
  • [18] Study on the neuroprotective effect of fluoxetine against MDMA-induced neurotoxicity on the serotonin transporter in rat brain using micro-PET
    Li, I-Hsun
    Huang, Wen-Sheng
    Shiue, Chyng-Yann
    Huang, Ya-Yao
    Liu, Ren-Shyan
    Chyueh, San-Chong
    Hu, Shih-Hsiung
    Liao, Mei-Hsiu
    Shen, Lie-Hang
    Liu, Jiang-Chuan
    Ma, Kuo-Hsing
    [J]. NEUROIMAGE, 2010, 49 (02) : 1259 - 1270
  • [19] Cochlear synaptopathy in acquired sensorineural hearing loss: Manifestations and mechanisms
    Liberman, M. Charles
    Kujawa, Sharon G.
    [J]. HEARING RESEARCH, 2017, 349 : 138 - 147
  • [20] Lin KJ, 2004, J NUCL MED, V45, P673
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