Evaluation of brain SERT with 4-[18F]-ADAM/micro-PET and hearing protective effects of dextromethorphan in hearing loss rat model

被引：2
作者：
Liu, Cheng-Tsung [1 ,2 ]
Huang, Yuahn-Sieh [3 ]
Chen, Hsin-Chien [4 ]
Ma, Kuo-Hsing [3 ]
Wang, Chih-Hung [1 ,5 ]
Chiu, Chuang-Hsin [6 ]
Shih, Jui-Hu [2 ,7 ]
Kang, Hsiao-Hsien [8 ]
Shiue, Chyng-Yann [6 ]
Li, I-Hsun [1 ,2 ,7 ]
机构：
[1] Natl Def Med Ctr, Grad Inst Med Sci, Taipei, Taiwan
[2] Triserv Gen Hosp, Dept Pharm Practice, Taipei, Taiwan
[3] Natl Def Med Ctr, Dept Biol & Anat, Taipei, Taiwan
[4] Triserv Gen Hosp, Dept Otorhinolaryngol Head & Neck Surg, Taipei, Taiwan
[5] Taichung Armed Forces Gen Hosp, Dept Otorhinolcuyngol, Taichung, Taiwan
[6] Triserv Gen Hosp, Dept Nucl Med, Taipei, Taiwan
[7] Natl Def Med Ctr, Sch Pharm, Taipei, Taiwan
[8] Universal Eye Ctr, Taipei, Taiwan
来源：
TOXICOLOGY AND APPLIED PHARMACOLOGY | 2019年 / 378卷
关键词：
Dextromethorphan; 4-[F-18]-ADAM; SERT; Micro-PET; Noise-Induced Hearing Loss; SEROTONIN TRANSPORTER; PLASTICITY; NEUROTRANSMISSION; DAMAGE;
D O I：
10.1016/j.taap.2019.114604
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
This study investigated the protective effects of dextromethorphan (DXM) on noise-induced hearing loss (NIHL) in rats. This study aimed to improve the auditory threshold and to understand the protective effects of DXM against N-methyl-D-aspartate (NMDA)-induced neurite degeneration of serotonergic neurons. The animals were exposed to 8-kHz narrowband noise at a 118-dB sound pressure level for 3.5 h. The hearing thresholds were determined by measuring the auditory brainstem response to click stimuli. Serotonin transporter (SERT) expression was determined through micro-positron emission tomography (PET) using N,N-dimethyl-2-(2-amino-4-F-18-fluorophenylthio)benzylamine (4-[F-18]-ADAM). We also investigated the effects of DXM on NMDA-induced morphological changes in the primary cultures of rat serotonergic neurons. NIHL significantly improved after prophylactic treatment with DXM (p < .05). SERT density in DXM-treated rats was significantly higher than that in non-DXM-treated rats. Because prophylactic medication restored the NMDA-inhibited neurite length of serotonergic neurons and presented SEAT density, DXM could be a potential agent in alleviating NIHL.
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页数：10
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共 42 条
  • [1] Replicates and repeats
    Bell, Graham
    [J]. BMC BIOLOGY, 2016, 14
  • [2] Effect of the Environment on the Dendritic Morphology of the Rat Auditory Cortex
    Bose, Mitali
    Munoz-Llancao, Pablo
    Roychowdhury, Swagata
    Nichols, Justin A.
    Jakkamsetti, Vikram
    Porter, Benjamin
    Byrapureddy, Rajasekhar
    Salgado, Humberto
    Kilgard, Michael P.
    Aboitiz, Francisco
    Dagnino-Subiabre, Alexies
    Atzori, Marco
    [J]. SYNAPSE, 2010, 64 (02) : 97 - 110
  • [3] CONFORMATIONALLY DEFINED NEUROTRANSMITTER ANALOGS - SELECTIVE-INHIBITION OF GLUTAMATE UPTAKE BY ONE PYRROLIDINE-2,4-DICARBOXYLATE DIASTEREOMER
    BRIDGES, RJ
    STANLEY, MS
    ANDERSON, MW
    COTMAN, CW
    CHAMBERLIN, AR
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 1991, 34 (02) : 717 - 725
  • [4] Reactive oxygen radicals in signaling and damage in the ischemic brain
    Chan, PH
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2001, 21 (01) : 2 - 14
  • [5] EXCITOTOXIC CELL-DEATH
    CHOI, DW
    [J]. JOURNAL OF NEUROBIOLOGY, 1992, 23 (09): : 1261 - 1276
  • [6] Specific sound-induced noradrenergic and serotonergic activation in central auditory structures
    Cransac, H
    Cottet-Emard, JM
    Hellström, S
    Peyrin, L
    [J]. HEARING RESEARCH, 1998, 118 (1-2) : 151 - 156
  • [7] Experimental design and analysis and their reporting II: updated and simplified guidance for authors and peer reviewers
    Curtis, Michael J.
    Alexander, Steve
    Cirino, Giuseppe
    Docherty, James R.
    George, Christopher H.
    Giembycz, Mark A.
    Hoyer, Daniel
    Insel, Paul A.
    Izzo, Angelo A.
    Ji, Yong
    MacEwan, David J.
    Sobey, Christopher G.
    Stanford, S. Clare
    Teixeira, Mauro M.
    Wonnacott, Sue
    Ahluwalia, Amrita
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 2018, 175 (07) : 987 - 993
  • [8] Significance of Serotonin Transporter Gene Polymorphism in Tinnitus
    Deniz, Murat
    Bayazit, Yildirim A.
    Celenk, Fatih
    Karabulut, Hayriye
    Yilmaz, Akin
    Gunduz, Bulent
    Saridogan, Cagil
    Dagli, Muharrerm
    Erdal, Emin
    Menevse, Adnan
    [J]. OTOLOGY & NEUROTOLOGY, 2010, 31 (01) : 19 - 24
  • [9] MECHANISM OF KAINATE TOXICITY TO CEREBELLAR NEURONS INVITRO IS ANALOGOUS TO REPERFUSION TISSUE-INJURY
    DYKENS, JA
    STERN, A
    TRENKNER, E
    [J]. JOURNAL OF NEUROCHEMISTRY, 1987, 49 (04) : 1222 - 1228
  • [10] Acquired hearing loss and brain plasticity
    Eggermont, Jos J.
    [J]. HEARING RESEARCH, 2017, 343 : 176 - 190
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