Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen

被引：67

作者：

Heimerman, Mallory E. ^{[1
,4
]}

Murgia, Maria V. ^{[1
,5
]}

Wu, Ping ^{[2
]}

Lowe, Andre D. ^{[2
,3
]}

Jia, Wei ^{[2
]}

Rowland, Raymond R. ^{[1
]}

机构：

[1] Kansas State Univ, Coll Vet Med, Diagnost Med & Pathobiol, Manhattan, KS 66506 USA

[2] USDA, Foreign Anim Dis Diagnost Lab, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv,Plum Isl Anim Dis, New York, NY USA

[3] Natl Emerging Infect Dis Labs, Boston, MA USA

[4] Thermo Fisher Sci, Lenexa, KS USA

[5] Purdue Univ, W Lafayette, IN 47907 USA

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2018年 / 30卷 / 03期

关键词：

African swine fever virus; monoclonal antibodies; p72; LINKED-IMMUNOSORBENT-ASSAY; STRUCTURAL PROTEIN P72; IDENTIFICATION;

D O I：

10.1177/1040638717753966

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E. coli and oligopeptides. Based on oligopeptide recognition, the mAbs were divided into 4 groups: mAb 85 (aa 165-171); mAbs 65-3 and 6H9-1 (aa 265-280); mAbs 8F7-3 and 23 (aa 280-294); and mAb 4A4 (aa 290-303). All mAbs were located within a highly conserved region in p72. This panel of antibodies provides the opportunity to develop new assays for the detection of ASFV antibody and antigen.

引用

页码：406 / 412

页数：7

共 17 条

[11] Molecular epidemiology of African swine fever in East Africa [J].

Lubisi, BA ;

Bastos, ADS ;

Dwarka, RM ;

Vosloo, W .

ARCHIVES OF VIROLOGY, 2005, 150 (12) :2439-2452

[12]

PASTOR MJ, 1990, AM J VET RES, V51, P1540

[13] African swine fever virus morphogenesis [J].

Salas, Maria L. ;

Andres, German .

VIRUS RESEARCH, 2013, 173 (01) :29-41

[14] MONOCLONAL-ANTIBODIES SPECIFIC FOR AFRICAN SWINE FEVER VIRUS PROTEINS [J].

SANZ, A ;

GARCIABARRENO, B ;

NOGAL, ML ;

VINUELA, E ;

ENJUANES, L .

JOURNAL OF VIROLOGY, 1985, 54 (01) :199-206

[15] Development of a novel lateral flow assay for detection of African swine fever in blood [J].

Sastre, P. ;

Gallardo, C. ;

Monedero, A. ;

Ruiz, T. ;

Arias, M. ;

Sanz, A. ;

Rueda, P. .

BMC VETERINARY RESEARCH, 2016, 12

[16] Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical swine fever viruses [J].

Sastre, Patricia ;

Perez, Teresa ;

Costa, Sofia ;

Yang, Xiaoping ;

Raeber, Alex ;

Blome, Sandra ;

Goller, Katja V. ;

Gallardo, Carmina ;

Tapia, Istar ;

Garcia, Julia ;

Sanz, Antonio ;

Rueda, Paloma .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2016, 28 (05) :543-549

[17] A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of African swine fever virus antigens and antibodies [J].

Vidal, MI ;

Stiene, M ;

Henkel, J ;

Bilitewski, U ;

Costa, JV ;

Oliva, AG .

JOURNAL OF VIROLOGICAL METHODS, 1997, 66 (02) :211-218

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