Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

被引：35
作者：
D'Alvise, Janina [1 ]
Mortensen, Rasmus [2 ]
Hansen, Steen H. [1 ]
Janfelt, Christian [1 ]
机构：
[1] Univ Copenhagen, Dept Pharm, DK-2100 Copenhagen, Denmark
[2] LEO Pharma AS, Pharmaceut Technol, DK-2750 Ballerup, Denmark
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2014年 / 406卷 / 15期
关键词：
DESI; Lidocaine; Mass spectrometry imaging; Pig ear skin; Drugs; Topical; DESORPTION ELECTROSPRAY-IONIZATION; IN-VIVO METABOLISM; HAIR-FOLLICLES; DRUG-DELIVERY; VITRO; MODEL; PERMEATION; THICKNESS; RESERVOIR; SECTIONS;
D O I：
10.1007/s00216-014-7802-z
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies.
引用
收藏
页码：3735 / 3742
页数：8
相关论文
共 49 条
[1]   The effects of freezing skin on transdermal drug penetration kinetics [J].
Ahlstrom, L. A. ;
Cross, S. E. ;
Mills, P. C. .
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 2007, 30 (05) :456-463
[2]   Matrix-Assisted Laser Desorption Ionization Imaging Mass Spectrometry: In Situ Molecular Mapping [J].
Angel, Peggi M. ;
Caprioli, Richard M. .
BIOCHEMISTRY, 2013, 52 (22) :3818-3828
[3]   Transdermal Drug Delivery In Vitro Using Diffusion Cells [J].
Bartosova, L. ;
Bajgar, J. .
CURRENT MEDICINAL CHEMISTRY, 2012, 19 (27) :4671-4677
[4]   In vitro 3D Full-Thickness Skin-Equivalent Tissue Model Using Silk and Collagen Biomaterials [J].
Bellas, Evangelia ;
Seiberg, Miri ;
Garlick, Jonathan ;
Kaplan, David L. .
MACROMOLECULAR BIOSCIENCE, 2012, 12 (12) :1627-1636
[5]   Improved spatial resolution in the imaging of biological tissue using desorption electrospray ionization [J].
Campbell, Dahlia I. ;
Ferreira, Christina R. ;
Eberlin, Livia S. ;
Cooks, R. Graham .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2012, 404 (02) :389-398
[6]   Comparative Pharmacokinetic Assessments of Topical Drugs: Evaluation by Dermatopharmacokinetics, Microdialysis, and Systemic Measurement [J].
Chik, Zamri ;
Tucker, Arthur T. ;
Shiel, Julian I. ;
Collier, David J. ;
Perrett, David ;
Lee, Terrence D. ;
Johnston, Atholl .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2010, 130 (12) :2828-2830
[7]   INVIVO METABOLISM OF LIDOCAINE IN THE RAT - ISOLATION OF URINARY METABOLITES AS PENTAFLUOROBENZOYL DERIVATIVES AND THEIR IDENTIFICATION BY COMBINED GAS CHROMATOGRAPHY-MASS SPECTROMETRY [J].
COUTTS, RT ;
TOROKBOTH, GA ;
CHU, LV ;
TAM, YK ;
PASUTTO, FM .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 421 (02) :267-280
[8]   Xenobiotic-Metabolizing Cytochromes P450 in Human White Adipose Tissue: Expression and Induction [J].
Ellero, Sandrine ;
Chakhtoura, Ghassan ;
Barreau, Corinne ;
Langouet, Sophie ;
Benelli, Chantal ;
Penicaud, Luc ;
Beaune, Philippe ;
de Waziers, Isabelle .
DRUG METABOLISM AND DISPOSITION, 2010, 38 (04) :679-686
[9]   Improved sample preparation for MALDI-MSI of endogenous compounds in skin tissue sections and mapping of exogenous active compounds subsequent to ex-vivo skin penetration [J].
Enthaler, Bernd ;
Pruns, Julia K. ;
Wessel, Sonja ;
Rapp, Claudius ;
Fischer, Markus ;
Wittern, Klaus-Peter .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2012, 402 (03) :1159-1167
[10]   Assessment of Near-Infrared Densitometry for in situ Determination of the Total Stratum Corneum Thickness on Pig Skin: Influence of Storage Time [J].
Franzen, L. ;
Windbergs, M. ;
Hansen, S. .
SKIN PHARMACOLOGY AND PHYSIOLOGY, 2012, 25 (05) :249-256
12345