RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZED PRODUCTION OF AN ALKALOPHILIC LACCASE FROM γ-PROTEOBACTERIUM JB

被引：0

作者：

Singh, Gursharan ^{[1
]}

Ahuja, Naveen ^{[2
]}

Sharma, Prince ^{[1
]}

Capalash, Neena ^{[3
]}

机构：

[1] Panjab Univ, Dept Microbiol, Chandigarh 160014, India

[2] Panjab Univ, Univ Inst Pharmaceut Sci, Chandigarh 160014, India

[3] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

来源：

BIORESOURCES | 2009年 / 4卷 / 02期

关键词：

Alkalophilic laccase; Production; RSM; gamma-proteobacterium JB; PURIFICATION; PARAMETERS; INDUSTRIAL; INDUCTION; PULP;

D O I：

10.15376/biores.4.2.544-553

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

gamma-Proteobacterium JB, an alkali-tolerant soil isolate, produced laccase (8X10(3) nkat/L) in M162 medium. The optimization of process conditions (pH, incubation time, agitation, and CuSO4 concentration) for laccase production during submerged fermentation was carried out using response surface methodology (RSM) based on a central composite design (CCD). Maximum laccase production achieved was 7.4 X 10(4) nkat/L at pH 8.0, 210 rpm, 100 PM Of CuSO4 after 60 h of incubation. This design of experiment methodology increased laccase production by 9.3 fold over the control. Experimental findings were in close agreement with the model predictions.

引用

页码：544 / 553

页数：10

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