A graphene oxide-based fluorescent sensor for recognition of glutamate in aqueous solutions and bovine serum

被引：2
作者：
Cheng, Rumei [1 ]
Cheng, Lingling [2 ]
Ou, Shengju [3 ]
机构：
[1] Wenzhou Med Univ, Sch Ophthalmol & Optometry, 270 Xueyuan Rd, Wenzhou 325027, Peoples R China
[2] Zhejiang Prov Peoples Hosp, Nursing Dept, Hangzhou, Zhejiang, Peoples R China
[3] Zhejiang Univ, Hangzhou Zheda Femtosecond Test Technol Co Ltd, Natl Sci Pk, Hangzhou, Zhejiang, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2019年 / 221卷
基金：
中国国家自然科学基金;
关键词：
Graphene oxide; Fluorescent sensor; Glutamate; Serum; Dynamic quenching; RESONANCE ENERGY-TRANSFER; PERFORMANCE; ADSORPTION; ACIDS;
D O I：
10.1016/j.saa.2019.117204
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A novel fluorescence probe based on graphene-aminofluorescein (GAF) for sensing glutamate is prepared by modifying graphene oxide (GO) with 5-aminofluorescein (AF), and shows high sensitivity and selectivity. The strong fluorescence of the GAF probe is quenched in the presence of glutamate, and the quenching exhibits a good linear relationship with the glutamate concentration within the range of 1-45 mg/L. In bovine serum, the accurate quantitation of glutamate is possible within the range of 6 mg/L to 30 mg/L. At the pH of 3.32 (close to the isoelectric point of glutamate), GAF can selectively detect glutamate in preference to other amino acids. The high sensitivity and specificity of this sensor enable a new method for the detection of glutamate in aqueous solutions and serums. (C) 2019 Elsevier B.V. All rights reserved.
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页数：6
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