Multiple-capillary measurement of RBC speed, flux, and density with optical coherence tomography

被引：58
作者：
Lee, Jonghwan [1 ]
Wu, Weicheng [1 ]
Lesage, Frederic [2 ]
Boas, David A. [1 ]
机构：
[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Martinos Ctr Biomed Imaging, Charlestown, MA 02129 USA
[2] Polytech Montreal, Dept Elect Engn, Montreal, PQ, Canada
来源：
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2013年 / 33卷 / 11期
关键词：
capillaries; cerebral blood flow measurement; cranial windows; microscopy; optical imaging; STIMULUS-INDUCED CHANGES; BLOOD-FLOW; INDIVIDUAL CAPILLARIES; FLUCTUATIONS; PERFUSION; VELOCITY; CORTEX;
D O I：
10.1038/jcbfm.2013.158
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
As capillaries exhibit heterogeneous and fluctuating dynamics even during baseline, a technique measuring red blood cell (RBC) speed and flux over many capillaries at the same time is needed. Here, we report that optical coherence tomography can capture individual RBC passage simultaneously over many capillaries located at different depths. Further, we demonstrate the ability to quantify RBC speed, flux, and linear density. This technique will provide a means to monitor microvascular flow dynamics over many capillaries at different depths at the same time.
引用
收藏
页码：1707 / 1710
页数：4
相关论文
共 13 条
  • [1] OPTICAL COHERENCE TOMOGRAPHY
    HUANG, D
    SWANSON, EA
    LIN, CP
    SCHUMAN, JS
    STINSON, WG
    CHANG, W
    HEE, MR
    FLOTTE, T
    GREGORY, K
    PULIAFITO, CA
    FUJIMOTO, JG
    [J]. SCIENCE, 1991, 254 (5035) : 1178 - 1181
  • [2] VIDEO MICROSCOPY OF CEREBROCORTICAL CAPILLARY-FLOW - RESPONSE TO HYPOTENSION AND INTRACRANIAL HYPERTENSION
    HUDETZ, AG
    FEHER, G
    WEIGLE, CGM
    KNUESE, DE
    KAMPINE, JP
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1995, 268 (06): : H2202 - H2210
  • [3] Kamoun WS, 2010, NAT METHODS, V7, P655, DOI [10.1038/NMETH.1475, 10.1038/nmeth.1475]
  • [4] Megahertz OCT for ultrawide-field retinal imaging with a 1050nm Fourier domain mode-locked laser
    Klein, Thomas
    Wieser, Wolfgang
    Eigenwillig, Christoph M.
    Biedermann, Benjamin R.
    Huber, Robert
    [J]. OPTICS EXPRESS, 2011, 19 (04): : 3044 - 3062
  • [5] Cortical blood flow through individual capillaries in rat vibrissa S1 cortex: stimulus-induced changes in flow are comparable to the underlying fluctuations in flow
    Kleinfeld, D
    [J]. BRAIN ACTIVATION AND CBF CONTROL, PROCEEDINGS, 2002, 1235 : 115 - 122
  • [6] Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex
    Kleinfeld, D
    Mitra, PP
    Helmchen, F
    Denk, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) : 15741 - 15746
  • [7] Quantitative imaging of cerebral blood flow velocity and intracellular motility using dynamic light scattering-optical coherence tomography
    Lee, Jonghwan
    Radhakrishnan, Harsha
    Wu, Weicheng
    Daneshmand, Ali
    Climov, Mihail
    Ayata, Cenk
    Boas, David A.
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2013, 33 (06) : 819 - 825
  • [8] Motion correction for phase-resolved dynamic optical coherence tomography imaging of rodent cerebral cortex
    Lee, Jonghwan
    Srinivasan, Vivek
    Radhakrishnan, Harsha
    Boas, David A.
    [J]. OPTICS EXPRESS, 2011, 19 (22): : 21258 - 21270
  • [9] Cortical electrical stimulation alters erythrocyte perfusion pattern in the cerebral capillary network of the rat
    Schulte, ML
    Wood, JD
    Hudetz, AG
    [J]. BRAIN RESEARCH, 2003, 963 (1-2) : 81 - 92
  • [10] Rapid volumetric angiography of cortical microvasculature with optical coherence tomography
    Srinivasan, Vivek J.
    Jiang, James Y.
    Yaseen, Mohammed A.
    Radhakrishnan, Harsha
    Wu, Weicheng
    Barry, Scott
    Cable, Alex E.
    Boas, David A.
    [J]. OPTICS LETTERS, 2010, 35 (01) : 43 - 45
12