Optimization of protease production from newly isolated strain of Bacillus sp PCSIR EA-3

被引：0

作者：

Ul Qadar, Shah Ali ^{[1
]}

Shireen, Erum ^{[2
]}

Iqbal, Samina ^{[3
]}

Anwar, Abida ^{[3
]}

机构：

[1] Univ Karachi, Inst Sustainable Halophyte Utilizat, Karachi, Pakistan

[2] Univ Karachi, Dept Biochem, Karachi, Pakistan

[3] Pharmaceut Res Ctr, Karachi, Pakistan

来源：

INDIAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 03期

关键词：

Bacillus sp; calcium ion; culture optimization; protease; ALKALINE PROTEASE; FERMENTATION; PURIFICATION;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A newly isolated strain of Bacillus sp. PCSIR EA-3 showed maximum protease production in 48 h. Cell free filtrate (CFF) from the strain was investigated for its protcolytic activity. The isolated strain exhibited highest proteolytic activity when grown on skim milk agar; average area of zone of inhibition was 480 mm(2). Enzyme showed maximum activity at 35 degrees C and pH 7.0 with glucose as an important medium component. It was strongly activated by metal ions, such as, Ca+2 ion. Protease from PCSIR EA-3 can easily be used in food industry, especially in cheese manufacturing due to its thermostabilily at neutral pH.

引用

页码：286 / 290

页数：5

共 33 条

[1]

Adinarayana K, 2002, J PHARM PHARM SCI, V5, P281

[2] SOAPS AND DETERGENTS [J].

AINSWORTH, SJ .

CHEMICAL & ENGINEERING NEWS, 1994, 72 (04) :34-&

[3]

Andrade VS, 2002, BRAZ J MICROBIOL, V33, P106, DOI 10.1590/S1517-83822002000200002

[4] The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin [J].

Anson, ML .

JOURNAL OF GENERAL PHYSIOLOGY, 1938, 22 (01) :79-89

[5]

BARRETT AJ, 1994, METHOD ENZYMOL, V244, P1

[6] THE MOLECULAR EVOLUTION OF GENES AND PROTEINS - A TALE OF 2 SERINES [J].

BRENNER, S .

NATURE, 1988, 334 (6182) :528-530

[7]

Chantawannkul P., 2002, Science Asia, V28, P241

[8] PRODUCTION AND PROPERTIES OF AN ALKALINE PROTEASE BY AUREOBASIDIUM-PULLULANS [J].

DONAGHY, JA ;

MCKAY, AM .

JOURNAL OF APPLIED BACTERIOLOGY, 1993, 74 (06) :662-666

[9]

Ellaiah P., 2002, Indian Journal of Microbiology, V42, P173

[10]

Gajju H., 1996, IND J MICROBIOL, V36, P153

← 1 2 3 4 →