Identification of white truffle species using RAPD markers

被引：6
|
作者：
Rossi, I [1 ]
Bartolacci, B [1 ]
Potenza, L [1 ]
Bertini, L [1 ]
Barbieri, E [1 ]
Stocchi, V [1 ]
机构：
[1] Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, PS, Italy
来源：
PLANT AND SOIL | 2000年 / 219卷 / 1-2期
关键词：
fruit body-ectomycorrhiza-mycelium; RAPD analysis; white truffles;
D O I：
10.1023/A:1004792528164
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Morphologically very similar species of white truffle were analyzed by the random amplified polymorphic DNA (RAPD) technique. Species-specific RAPD fragments were selected and pairs of primers were designed on their sequences. Sequence-characterized amplified regions were developed and applied to identify these species throughout their entire life cycle: fruit body, mycelium, ectomycorrhiza. This procedure provides an unambiguous and rapid tool for typing species whose morphology is very similar.
引用
收藏
页码：127 / 133
页数：7
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