Frequent promoter hypermethylation and transcriptional downregulation of BTG4 gene in gastric cancer

被引：24
作者：
Dong, Wenjie
Tu, Shuiping [2 ]
Xie, Jing [3 ]
Sun, Pinghu
Wu, Yunlin
Wang, Lifu [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Rui Jin Hosp, Sch Med, Dept Gastroenterol, Shanghai 200025, Peoples R China
[2] Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY 10027 USA
[3] Shanghai Jiao Tong Univ, Rui Jin Hosp, Dept Pathol, Shanghai 200025, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 387卷 / 01期
基金：
中国国家自然科学基金;
关键词：
BTG4; Gastric cancer; Hypermethylation; HETEROZYGOSITY; METHYLATION; IDENTIFICATION; EXPRESSION; CARCINOMA; ADENOCARCINOMA; SUPPRESSION; MARKER; REGION; 11Q23;
D O I：
10.1016/j.bbrc.2009.06.140
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The BTG4 gene belongs to the BTG family of genes endowed with antiproliferative properties. In this Study, we have found that BTG4 undergoes promoter CpG island hypermethylation-associated inactivation in gastric cancer and 5'-aza-2'-deoxycytidine (DAC) treatment restores BTG4 expression. We also found BTG4 levels were significantly reduced in primary gastric cancer but not ill normal gastric tissues. BTG4 reexpression in gastric cancer causes growth inhibition of colony assays and mucle mice. Taken together, our data support BTG4 as a candidate tumor suppressor gene that is epigenetically silenced in the majority of gastric cancers. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：132 / 138
页数：7
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