A case of Mycobacterium tuberculosis laboratory cross-contamination

被引：4

作者：

Takeda, Keita ^{[1
,2
,5
]}

Murase, Yoshiro ^{[1
]}

Kawashima, Masahiro ^{[2
]}

Suzukawa, Maho ^{[3
]}

Suzuki, Junko ^{[2
]}

Yamane, Akira ^{[2
]}

Igarashi, Yuriko ^{[1
]}

Chikamatsu, Kinuyo ^{[1
]}

Morishige, Yuta ^{[1
]}

Aono, Akio ^{[1
]}

Yamada, Hiroyuki ^{[1
]}

Takaki, Akiko ^{[1
]}

Tamura, Atsuhisa ^{[2
]}

Nagai, Hideaki ^{[2
]}

Matsui, Hirotoshi ^{[2
]}

Tohma, Shigeto ^{[4
]}

Mitarai, Satoshi ^{[1
,5
]}

机构：

[1] Res Inst TB, Dept Mycobacterium Reference & Res, Tokyo, Japan

[2] Natl Hosp Org Tokyo Natl Hosp, Ctr Pulm Dis, 3-1-1 Takeoka, Tokyo 2048585, Japan

[3] Natl Hosp Org Tokyo Natl Hosp, Clin Res Ctr, Tokyo, Japan

[4] Natl Hosp Org Tokyo Natl Hosp, Asthma Allergy & Rheumatol Ctr, Tokyo, Japan

[5] Nagasaki Univ, Grad Sch Biomed Sci, Dept Basic Mycobacteriol, Nagasaki, Japan

来源：

JOURNAL OF INFECTION AND CHEMOTHERAPY | 2019年 / 25卷 / 08期

基金：

日本学术振兴会;

关键词：

Variable number of tandem repeat; False-positive results; Mycobacterium tuberculosis; NUMBER-TANDEM-REPEAT; FALSE-POSITIVE CULTURES; VARIABLE NUMBERS; IS6110; LOCI;

D O I：

10.1016/j.jiac.2019.03.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Setting: A laboratory cross-contamination event was suspected because Mycobacterium tuberculosis was unexpectedly detected at a high incidence in the cultures of several clinical specimens at the National Hospital Organization, Tokyo National Hospital, Japan. Objective: To describe a case of Mycobacterium tuberculosis laboratory cross-contamination. Design: We reviewed the medical records of 20 patients whose clinical specimens were suspected to have been contaminated by Mycobacterium tuberculosis. Variable number of tandem repeat analysis with 15 loci, the Japan Anti-Tuberculosis Association-12, and three additional hyper-variable loci, was performed to identify the cross-contamination event. Results: The clinical, laboratory, and variable number of tandem repeat data revealed that the cross-contamination had possibly originated from one strongly positive specimen, resulting in false-positive results in 11 other specimens, including a case treated with anti-tuberculosis drugs. Conclusion: Clinical and laboratory data must be re-evaluated when cross-contamination is suspected and variable number of tandem repeat analysis should be used to confirm cross-contamination. Furthermore, original isolates should be stored appropriately, without sub-culturing and genotyping should be performed at the earliest possible for better utilization of variable number of tandem repeat for the identification of cross-contamination. (c) 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：610 / 614

页数：5

共 23 条

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