An effector from the Huanglongbing-associated pathogen targets citrus proteases

被引:145
作者
Clark, Kelley [1 ]
Franco, Jessica Yvette [2 ]
Schwizer, Simon [1 ,3 ]
Pang, Zhiqian [4 ]
Hawara, Eva [1 ]
Liebrand, Thomas W. H. [2 ]
Pagliaccia, Deborah [1 ]
Zeng, Liping [1 ,3 ]
Gurung, Fatta B. [5 ]
Wang, Pengcheng [6 ]
Shi, Jinxia [1 ,8 ]
Wang, Yinsheng [6 ]
Ancona, Veronica [5 ]
van der Hoorn, Renier A. L. [7 ]
Wang, Nian [4 ]
Coaker, Gitta [2 ]
Ma, Wenbo [1 ,3 ]
机构
[1] Univ Calif Riverside, Dept Microbiol & Plant Pathol, Riverside, CA 92521 USA
[2] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
[3] Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA
[4] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA
[5] Texas A&M Univ, Citrus Ctr, Weslaco, TX 78599 USA
[6] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA
[7] Univ Oxford, Oxford OX1 2JD, England
[8] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China
基金
美国食品与农业研究所;
关键词
CANDIDATUS LIBERIBACTER ASIATICUS; PHYTOPHTHORA-INFESTANS; CYSTEINE PROTEASES; VIRULENCE FACTOR; PLANT; DISEASE; DEFENSE; COMPLEX; IDENTIFICATION; INHIBITORS;
D O I
10.1038/s41467-018-04140-9
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The citrus industry is facing an unprecedented challenge from Huanglongbing (HLB). All cultivars can be affected by the HLB-associated bacterium 'Candidatus Liberibacter asiaticus' (CLas) and there is no known resistance. Insight into HLB pathogenesis is urgently needed in order to develop effective management strategies. Here, we use Sec-delivered effector 1 (SDE1), which is conserved in all CLas isolates, as a molecular probe to understand CLas virulence. We show that SDE1 directly interacts with citrus papain-like cysteine proteases (PLCPs) and inhibits protease activity. PLCPs are defense-inducible and exhibit increased protein accumulation in CLas-infected trees, suggesting a role in citrus defense responses. We analyzed PLCP activity in field samples, revealing specific members that increase in abundance but remain unchanged in activity during infection. SDE1-expressing transgenic citrus also exhibit reduced PLCP activity. These data demonstrate that SDE1 inhibits citrus PLCPs, which are immune-related proteases that enhance defense responses in plants.
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页数:11
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