An effector from the Huanglongbing-associated pathogen targets citrus proteases

被引：159

作者：

Clark, Kelley ^{[1
]}

Franco, Jessica Yvette ^{[2
]}

Schwizer, Simon ^{[1
,3
]}

Pang, Zhiqian ^{[4
]}

Hawara, Eva ^{[1
]}

Liebrand, Thomas W. H. ^{[2
]}

Pagliaccia, Deborah ^{[1
]}

Zeng, Liping ^{[1
,3
]}

Gurung, Fatta B. ^{[5
]}

Wang, Pengcheng ^{[6
]}

Shi, Jinxia ^{[1
,8
]}

Wang, Yinsheng ^{[6
]}

Ancona, Veronica ^{[5
]}

van der Hoorn, Renier A. L. ^{[7
]}

Wang, Nian ^{[4
]}

Coaker, Gitta ^{[2
]}

Ma, Wenbo ^{[1
,3
]}

机构：

[1] Univ Calif Riverside, Dept Microbiol & Plant Pathol, Riverside, CA 92521 USA

[2] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

[3] Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA

[4] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA

[5] Texas A&M Univ, Citrus Ctr, Weslaco, TX 78599 USA

[6] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

[7] Univ Oxford, Oxford OX1 2JD, England

[8] Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

美国食品与农业研究所;

关键词：

CANDIDATUS LIBERIBACTER ASIATICUS; PHYTOPHTHORA-INFESTANS; CYSTEINE PROTEASES; VIRULENCE FACTOR; PLANT; DISEASE; DEFENSE; COMPLEX; IDENTIFICATION; INHIBITORS;

D O I：

10.1038/s41467-018-04140-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The citrus industry is facing an unprecedented challenge from Huanglongbing (HLB). All cultivars can be affected by the HLB-associated bacterium 'Candidatus Liberibacter asiaticus' (CLas) and there is no known resistance. Insight into HLB pathogenesis is urgently needed in order to develop effective management strategies. Here, we use Sec-delivered effector 1 (SDE1), which is conserved in all CLas isolates, as a molecular probe to understand CLas virulence. We show that SDE1 directly interacts with citrus papain-like cysteine proteases (PLCPs) and inhibits protease activity. PLCPs are defense-inducible and exhibit increased protein accumulation in CLas-infected trees, suggesting a role in citrus defense responses. We analyzed PLCP activity in field samples, revealing specific members that increase in abundance but remain unchanged in activity during infection. SDE1-expressing transgenic citrus also exhibit reduced PLCP activity. These data demonstrate that SDE1 inhibits citrus PLCPs, which are immune-related proteases that enhance defense responses in plants.

引用

页数：11

共 60 条

[21] An Effector-Targeted Protease Contributes to Defense against Phytophthora infestans and Is under Diversifying Selection in Natural Hosts [J].

Kaschani, Farnusch ;

Shabab, Mohammed ;

Bozkurt, Tolga ;

Shindo, Takayuki ;

Schornack, Sebastian ;

Gu, Christian ;

Ilyas, Muhammad ;

Win, Joe ;

Kamoun, Sophien ;

van der Hoorn, Renier A. L. .

PLANT PHYSIOLOGY, 2010, 154 (04) :1794-1804

[22] Response of Sweet Orange (Citrus sinensis) to 'Candidatus Liberibacter asiaticus' Infection: Microscopy and Microarray Analyses [J].

Kim, Jeong-Soon ;

Sagaram, Uma Shankar ;

Burns, Jacqueline K. ;

Li, Jian-Liang ;

Wang, Nian .

PHYTOPATHOLOGY, 2009, 99 (01) :50-57

[23] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[24] SMART: recent updates, new developments and status in 2015 [J].

Letunic, Ivica ;

Doerks, Tobias ;

Bork, Peer .

NUCLEIC ACIDS RESEARCH, 2015, 43 (D1) :D257-D260

[25] Comparative proteomics of cucurbit phloem indicates both unique and shared sets of proteins [J].

Lopez-Cobollo, Rosa M. ;

Filippis, Ioannis ;

Bennett, Mark H. ;

Turnbull, Colin G. N. .

PLANT JOURNAL, 2016, 88 (04) :633-647

[26] Dual disease resistance mediated by the immune receptor Cf-2 in tomato requires a common virulence target of a fungus and a nematode [J].

Lozano-Torres, Jose L. ;

Wilbers, Ruud H. P. ;

Gawronski, Piotr ;

Boshoven, Jordi C. ;

Finkers-Tomczak, Anna ;

Cordewener, Jan H. G. ;

America, Antoine H. P. ;

Overmars, Hein A. ;

Van 't Klooster, John W. ;

Baranowski, Lukasz ;

Sobczak, Miroslaw ;

Ilyas, Muhammad ;

van der Hoorn, Renier A. L. ;

Schots, Arjen ;

de Wit, Pierre J. G. M. ;

Bakker, Jaap ;

Goverse, Aska ;

Smant, Geert .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (25) :10119-10124

[27] Phytoplasma Effector SAP54 Induces Indeterminate Leaf-Like Flower Development in Arabidopsis Plants [J].

MacLean, Allyson M. ;

Sugio, Akiko ;

Makarova, Olga V. ;

Findlay, Kim C. ;

Grieve, Victoria M. ;

Toth, Reka ;

Nicolaisen, Mogens ;

Hogenhout, Saskia A. .

PLANT PHYSIOLOGY, 2011, 157 (02) :831-841

[28] Transcriptome Profiling of Citrus Fruit Response to Huanglongbing Disease [J].

Martinelli, Federico ;

Uratsu, Sandra L. ;

Albrecht, Ute ;

Reagan, Russell L. ;

Phu, My L. ;

Britton, Monica ;

Buffalo, Vincent ;

Fass, Joseph ;

Leicht, Elizabeth ;

Zhao, Weixiang ;

Lin, Dawei ;

D'Souza, Raissa ;

Davis, Cristina E. ;

Bowman, Kim D. ;

Dandekar, Abhaya M. .

PLOS ONE, 2012, 7 (05)

[29] Papain-like cysteine proteases as hubs in plant immunity [J].

Misas-Villamil, Johana C. ;

van der Hoorn, Renier A. L. ;

Doehlemann, Gunther .

NEW PHYTOLOGIST, 2016, 212 (04) :902-907

[30] Improved real-time PCR detection of 'Candidatus Liberibacter asiaticus' from citrus and psyllid hosts by targeting the intragenic tandem-repeats of its prophage genes [J].

Morgan, J. Kent ;

Zhou, Lijuan ;

Li, Wenbin ;

Shatters, Robert G. ;

Keremane, Manjunath ;

Duan, Yong-Ping .

MOLECULAR AND CELLULAR PROBES, 2012, 26 (02) :90-98

← 1 2 3 4 5 6 →