A Magnetic Nanobead-Based Read-Out Procedure for Rapid Detection of DNA Molecules

被引：4

作者：

de la Torre, Teresa Zardan Gomez ^{[1
]}

Herthnek, David ^{[2
,3
]}

Stromme, Maria ^{[1
]}

机构：

[1] Uppsala Univ, Dept Engn Sci, S-75121 Uppsala, Sweden

[2] Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, S-17121 Stockholm, Sweden

[3] Uppsala Univ, Dept Immunol Genet & Pathol, Sci Life Lab, Rudbeck Lab, S-75121 Uppsala, Sweden

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2017年 / 17卷 / 04期

基金：

瑞典研究理事会;

关键词：

Brownian Relaxation; DNA Macromolecules; Magnetic Nanobeads; Rapid Detection; Rolling Circle Amplification; SENSITIVE DETECTION; BIOSENSORS; OLIGONUCLEOTIDES; NANOPARTICLES; REPLICATION; DIAGNOSTICS;

D O I：

10.1166/jnn.2017.13877

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The presented measurement and data analysis procedure reduces the read-out time for the volumeamplified magnetic nanobead detection assay from similar to 30 min to only 2 min, providing fast, sensitive detection of DNA molecules. The molecular detection and amplification protocol was verified using samples containing rolling circle-amplified DNA products formed from synthetic Vibrio cholerae target DNA, with a limit of detection of 5 pM. The developed read-out method could be used to rapidly identify pathogens in a variety of applications including target screening in hospitals with limited resources, in out-patient settings and in the field.

引用

页码：2861 / 2864

页数：4

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