Migration behavior of organic dyes based on physicochemical properties of solvents as background electrolytes in non-aqueous capillary electrophoresis

被引:6
作者
Gu, Minjeong [1 ]
Cho, Keunchang [2 ]
Kang, Seong Ho [1 ,3 ,4 ]
机构
[1] Kyung Hee Univ, Grad Sch, Dept Chem, Yongin 17104, Gyeonggi Do, South Korea
[2] Logos Biosyst Inc, Anyang Si 14059, Gyeonggi Do, South Korea
[3] Kyung Hee Univ, Dept Appl Chem, Yongin 17104, Gyeonggi Do, South Korea
[4] Kyung Hee Univ, Inst Nat Sci, Yongin 17104, Gyeonggi Do, South Korea
基金
新加坡国家研究基金会;
关键词
Organic dyes; Non-aqueous capillary electrophoresis; Migration behavior; Physicochemical properties; IONIZATION MASS-SPECTROMETRY; BALLPOINT PEN INKS; SEPARATION SELECTIVITY; ZONE-ELECTROPHORESIS; RAMAN-SPECTROSCOPY; VIOLET DYES; ION; MOBILITY; PHOTOISOMERIZATION; EQUILIBRIUM;
D O I
10.1016/j.chroma.2018.05.029
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The migration behavior of organic fluorescent dyes (i.e., crystal violet, methyl violet base, methyl violet B base, rhodamine 6G, and rhodamine B base) in non-aqueous capillary electrophoresis (NACE) was investigated by focusing on the physicochemical properties of various organic solvents [ethanol, methanol, 2-propanol, dimethylformamide (DMF), and dimethyl sulfoxide (DMSO)] in background electrolyte (BGE). Laser-induced fluorescence (LIF) and UV/Vis detectors were employed to observe both the migration time of organic dyes and the electroosmotic flow (EOF) in NACE, respectively. As seen in conventional aqueous BGE, the mobility of EOF in organic solvents tended to rise when the ratio between the dielectric constant and the solvent's viscosity (epsilon/eta) increased in accordance with Smoluchowski's equation. However, unlike the sin of pure organic solvents, the migration order of dyes changed as follows: methanol (60.0) > DMF (45.8) > ethanol (22.8) > DMSO (23.4) > 2-propanol (9.8). Since the amount of acetic acid added to balance the pH depends on the pK(a) a of each solvent, EOF changed when the difference in the (epsilon/eta) value was small. This resulted from the inhibition of mobility, and its difference was dependent on the (epsilon/eta) of BGEs with high ionic strength. In particular, the actual mobility of dyes in DMF showed excellent compliance with the Debye-Htickel-Onsager (DHO) theory extended by Falkenhagen and Pitts, which enabled us to analyze all dyes within 15 min with excellent resolution (R-s > 2.5) under optimum NACE conditions (10 mM sodium borate and 4661 mM acetic acid in 100% DMF, pH 4.5). In addition, the NACE method was successfully applied for analyzing commercially available ballpoint ink pens. Thus, these results could be used to anticipate the migration order of organic dyes in a 100% NACE separation system. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:82 / 90
页数:9
相关论文
共 56 条
[1]  
Andrasko J, 2001, J FORENSIC SCI, V46, P21
[2]   THE NATURE OF THE SILICA CAGE AS REFLECTED BY SPECTRAL CHANGES AND ENHANCED PHOTOSTABILITY OF TRAPPED RHODAMINE-6G [J].
AVNIR, D ;
LEVY, D ;
REISFELD, R .
JOURNAL OF PHYSICAL CHEMISTRY, 1984, 88 (24) :5956-5959
[3]   The effect of solvent grade on thin layer chromatographic analysis of writing inks [J].
Barker, Julia ;
Ramotowski, Robert ;
Nwokoye, Jennie .
FORENSIC SCIENCE INTERNATIONAL, 2016, 266 :139-147
[4]  
Bugler J.H., 2005, FORENSIC SCI, V50, DOI [10.1520/JFS2004362, DOI 10.1520/JFS2004362]
[5]   Analysis of questioned documents: A review [J].
Calcerrada, Matias ;
Garcia-Ruiz, Carmen .
ANALYTICA CHIMICA ACTA, 2015, 853 :143-166
[6]   SOLVENT EFFECTS ON THE PHOTOISOMERIZATION RATES OF THE ZWITTERIONIC AND THE CATIONIC FORMS OF RHODAMINE-B IN PROTIC SOLVENTS [J].
CHANG, TL ;
CHEUNG, HC .
JOURNAL OF PHYSICAL CHEMISTRY, 1992, 96 (12) :4874-4878
[7]   Ionic liquids: solvent properties and organic reactivity [J].
Chiappe, C ;
Pieraccini, D .
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY, 2005, 18 (04) :275-297
[8]   Direct Analysis of Textile Fabrics and Dyes Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometry [J].
Cochran, Kristin H. ;
Barry, Jeremy A. ;
Muddiman, David C. ;
Hinks, David .
ANALYTICAL CHEMISTRY, 2013, 85 (02) :831-836
[9]   Applications of capillary electrophoresis in forensic analytical chemistry [J].
Cruces-Blanco, C. ;
Gamiz-Gracia, L. ;
Garcia-Campana, A. M. .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2007, 26 (03) :215-226
[10]   Influence of electrolyte nature on the separation selectivity of amphetamines in nonaqueous capillary electrophoresis:: Protonation degree versus ion pairing effects [J].
Descroix, S ;
Varenne, A ;
Geiser, L ;
Cherkaoui, S ;
Veuthey, JL ;
Gareil, P .
ELECTROPHORESIS, 2003, 24 (10) :1577-1586