Design of studies using DNA microarrays

被引：119
作者：
Simon, R [1 ]
Radmacher, MD [1 ]
Dobbin, K [1 ]
机构：
[1] NCI, Biomet Res Branch, Bethesda, MD 20892 USA
来源：
GENETIC EPIDEMIOLOGY | 2002年 / 23卷 / 01期
关键词：
gene expression; bioinformatics; biomarkers; computational biology;
D O I：
10.1002/gepi.202
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA microarrays are assays that simultaneously provide information about expression levels of thousands of genes and are consequently finding wide use in biomedical research. In order to control the many sources of variation and the many opportunities for misanalysis, DNA microarray studies require careful planning. Different studies have different objectives, and important aspects of design and analysis strategy differ for different types of studies. We review several types of objectives of studies using DNA microarrays and address issues such as selection of samples, levels of replication needed, allocation of samples to dyes and arrays, sample size considerations, and analysis strategies. (C) 2002 Wiley-Liss, Inc.
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收藏
页码：21 / 36
页数：16
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