Biosensors for determination of total antioxidant capacity of phytotherapeutic integrators: comparison with other spectrophotometric, fluorimetric and voltammetric methods

被引:22
作者
Campanella, L [1 ]
Bonanni, A [1 ]
Bellantoni, D [1 ]
Tomassetti, M [1 ]
机构
[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
关键词
antioxidant capacity; evaluation; superoxide dismutase; biosensors; phytotherapeutic integrators;
D O I
10.1016/S0731-7085(03)00627-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzymatic electrodes based on superoxide dismutase (SOD) biosensors, working both in aqueous and non-aqueous solutions, recently developed by the present authors, were used to experimentally evaluate the antioxidant capacity of several phytotherapeutic diet integrators. The precision of this method of analysis was found to be reasonable (R.S.D. less than or equal to 10%). The results were also compared with those obtained using a traditional spectrophotometric method as well as a spectrofluorimetric method described in literature. Lastly, the comparison was extended to another method based on cyclic voltammetry currently being trialled by the present authors. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:303 / 320
页数:18
相关论文
共 25 条
[1]   A modified amperometric electrode for the determination of free radicals [J].
Campanella, L ;
Favero, G ;
Tomassetti, M .
SENSORS AND ACTUATORS B-CHEMICAL, 1997, 44 (1-3) :559-565
[2]   THE EFFECT OF ORGANIC-SOLVENT PROPERTIES ON THE RESPONSE OF A TYROSINASE ENZYME SENSOR [J].
CAMPANELLA, L ;
FAVERO, G ;
SAMMARTINO, MP ;
TOMASSETTI, M .
TALANTA, 1994, 41 (06) :1015-1023
[3]   Determination of the antioxidant capacity of samples of different types of tea, or of beverages based on tea or other herbal products, using a superoxide dismutase biosensor [J].
Campanella, L ;
Bonanni, A ;
Tomassetti, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 32 (4-5) :725-736
[4]   Determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor [J].
Campanella, L ;
Bonanni, A ;
Favero, G ;
Tomassetti, M .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 375 (08) :1011-1016
[5]   Evaluation of radical scavenging properties of several plants, fresh or from a herbalist's, using a superoxide dismutase biosensor [J].
Campanella, L ;
Favero, G ;
Persi, L ;
Tomassetti, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2001, 24 (5-6) :1055-1064
[6]   Superoxide dismutase biosensors working in non-aqueous solvent [J].
Campanella, L ;
De Luca, S ;
Favero, G ;
Persi, L ;
Tomassetti, M .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2001, 369 (7-8) :594-600
[7]   New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties [J].
Campanella, L ;
Favero, G ;
Persi, L ;
Tomassetti, M .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 23 (01) :69-76
[8]   The effect of organic solvent properties on a catalase enzyme sensor for monitoring hydrogen peroxide in nonaqueous solutions [J].
Campanella, L ;
Martini, U ;
Sammartino, MP ;
Tomassetti, M .
ELECTROANALYSIS, 1996, 8 (12) :1150-1154
[9]   Selective membrane sensors for free radical analysis based on potentiometric and CHEMFET devices [J].
Campanella, L ;
Favero, G ;
Occhionero, E ;
Tomassetti, M .
ANALUSIS, 1998, 26 (05) :223-228
[10]   Superoxide dismutase biosensors for superoxide radical analysis [J].
Campanella, L ;
Favero, G ;
Tomassetti, M .
ANALYTICAL LETTERS, 1999, 32 (13) :2559-2581