Effect of temperature shock on soybean microspore embryogenesis

被引：15
作者：
de Moraes, AP
Bonadese-Zanettini, MH
Callegari-Jacques, SM
Kaltchuk-Santos, E
机构：
[1] Univ Fed Rio Grande Sul, Dept Genet, BR-91501970 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande Sul, Dept Estatist, BR-91501970 Porto Alegre, RS, Brazil
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2004年 / 47卷 / 04期
关键词：
Anther culture; soybean; androgenesis; symmetrical mitosis; temperature shock; stress;
D O I：
10.1590/S1516-89132004000400006
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33degreesC, while the anthers used for control were maintained at 25degreesC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
引用
收藏
页码：537 / 544
页数：8
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