A promiscuous kinase inhibitor delineates the conspicuous structural features of protein kinase CK2a1

被引：3
作者：
Tsuyuguchi, Masato [1 ]
Nakaniwa, Tetsuko [2 ]
Sawa, Masaaki [3 ]
Nakanishi, Isao [4 ]
Kinoshita, Takayoshi [1 ]
机构：
[1] Osaka Prefecture Univ, Grad Sch Sci, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan
[2] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan
[3] Carna BioSci, Kobe, Hyogo 6500047, Japan
[4] Kindai Univ, Dept Pharmaceut Sci, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2019年 / 75卷
关键词：
protein kinase CK2; ATP-analogue inhibitor; selectivity; off-target kinases; structural comparison; CRYSTAL-STRUCTURE; IDENTIFICATION; CK2-ALPHA;
D O I：
10.1107/S2053230X19008951
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein kinase CK2a1 is a serine/threonine kinase that plays a crucial role in the growth, proliferation and survival of cells and is a well known target for tumour and glomerulonephritis therapies. Here, the crystal structure of the kinase domain of CK2a1 complexed with 5-iodotubercidin (5IOD), an ATP-mimetic inhibitor, was determined at 1.78 angstrom resolution. The structure shows distinct structural features and, in combination with a comparison of the crystal structures of five off-target kinases complexed with 5IOD, provides valuable information for the development of highly selective inhibitors.
引用
收藏
页码：515 / 519
页数：5
相关论文
共 22 条
  • [1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution
    Adams, Paul D.
    Afonine, Pavel V.
    Bunkoczi, Gabor
    Chen, Vincent B.
    Davis, Ian W.
    Echols, Nathaniel
    Headd, Jeffrey J.
    Hung, Li-Wei
    Kapral, Gary J.
    Grosse-Kunstleve, Ralf W.
    McCoy, Airlie J.
    Moriarty, Nigel W.
    Oeffner, Robert
    Read, Randy J.
    Richardson, David C.
    Richardson, Jane S.
    Terwilliger, Thomas C.
    Zwart, Peter H.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 : 213 - 221
  • [2] A General Framework for Inhibitor Resistance in Protein Kinases
    Balzano, Deborah
    Santaguida, Stefano
    Musacchio, Andrea
    Villa, Fabrizio
    [J]. CHEMISTRY & BIOLOGY, 2011, 18 (08): : 966 - 975
  • [3] Too much of a good thing: The role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2
    Duncan, James S.
    Litchfield, David W.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2008, 1784 (01): : 33 - 47
  • [4] Features and development of Coot
    Emsley, P.
    Lohkamp, B.
    Scott, W. G.
    Cowtan, K.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2010, 66 : 486 - 501
  • [5] Structure and functional characterization of the atypical human kinase haspin
    Eswaran, Jeyanthy
    Patnaik, Debasis
    Filippakopoulos, Panagis
    Wang, Fangwei
    Stein, Ross L.
    Murray, James W.
    Higgins, Jonathan M. G.
    Knapp, Stefan
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (48) : 20198 - 20203
  • [6] Halogen-Aromatic Interactions Modulate Inhibitor Residence Times
    Heroven, Christina
    Georgi, Victoria
    Ganotra, Gaurav K.
    Brennan, Paul
    Wolfreys, Finn
    Wade, Rebecca C.
    Fernandez-Montalvan, Amaury E.
    Chaikuad, Apirat
    Knapp, Stefan
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (24) : 7220 - 7224
  • [7] Second-generation CK2α inhibitors targeting the αD pocket
    Iegre, Jessica
    Brear, Paul
    De Fusco, Claudia
    Yoshida, Masao
    Mitchell, Sophie L.
    Rossmann, Maxim
    Carro, Laura
    Sore, Hannah F.
    Hyvonen, Marko
    Spring, David R.
    [J]. CHEMICAL SCIENCE, 2018, 9 (11) : 3041 - 3049
  • [8] Structure of human Fyn kinase domain complexed with staurosporine
    Kinoshita, Takayoshi
    Matsubara, Mamoru
    Ishiguro, Hiroshi
    Okita, Kouki
    Tada, Toshiji
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 346 (03) : 840 - 844
  • [9] Identification of allosteric ERK2 inhibitors through in silico biased screening and competitive binding assay
    Kinoshita, Takayoshi
    Sugiyama, Hajime
    Mori, Yurika
    Takahashi, Naruhide
    Tomonaga, Atsushi
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2016, 26 (03) : 955 - 958
  • [10] Crystal structure of human CK2α at 1.06 Å resolution
    Kinoshita, Takayoshi
    Nakaniwa, Tetsuko
    Sekiguchi, Yusuke
    Sogabe, Yuri
    Sakurai, Atsushi
    Nakamura, Shinya
    Nakanishi, Isao
    [J]. JOURNAL OF SYNCHROTRON RADIATION, 2013, 20 : 974 - 979
123