Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

被引：4

作者：

Liu, Getong ^{[1
]}

Huang, Xin ^{[1
]}

Pu, Qinlin ^{[1
]}

Zhao, Yongyun ^{[1
]}

Du, Feng ^{[1
]}

Dong, Juan ^{[1
]}

Cui, Xin ^{[1
]}

Tang, Zhuo ^{[1
]}

机构：

[1] Univ Chinese Acad Sci, Nat Prod Res Ctr, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 21期

基金：

中国科学院西部之光基金;

关键词：

IN-VITRO SELECTION; METAL-ION; CLEAVING RIBOZYMES; NUX RULE; CATALYSIS; SEQUENCE; RATES; SITE; PHOSPHOROTHIOATE; TRANSITION;

D O I：

10.1039/c7ob00995j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A new guideline for the construction of hammerhead ribozymes to achieve trans-cleavage of a single-stranded RNA molecule was developed. The sequence rule of the HHRz cleavage site was highly recommended to be "DWH" with an optimal binding arm length of 8-9nt, which diverged from the former rule of "NUX".

引用

页码：4681 / 4685

页数：5