Random amplified polymorphic DNA profiles as a tool for the characterization of Brazilian keratitis isolates of the genus Acanthamoeba

被引：36
作者：
Alves, JMP
Gusmao, CX
Teixeira, MMG
Freitas, D
Foronda, AS
Affonso, HT
机构：
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Oftalmol, Sao Paulo, Brazil
来源：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2000年 / 33卷 / 01期
关键词：
Acanthamoeba; keratitis; RAPD typing; genetic variation;
D O I：
10.1590/S0100-879X2000000100003
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The genus Acanthamoeba comprises free-living amebae identified as opportunistic pathogens of humans and other animal species. Morphological, biochemical and molecular approaches have shown wide genetic diversity within the genus. In an attempt to determine the genetic relatedness among isolates of Acanthamoeba we analyzed randomly amplified polymorphic DNA (RAPD) profiles of Il Brazilian isolates from cases of human keratitis and 8 American type culture collection (ATCC) reference strains. We found that ATCC strains belonging to the same species present polymorphic RAPD profiles whereas strains of different species show very similar profiles. Although most Brazilian isolates could not be assigned with certainty to any of the reference species, they could be clustered according to pattern similarities. The results show that RAPD analysis is a useful tool for the rapid characterization of new isolates and the assessment of genetic relatedness of Acanthamoeba spp. A comparison between RAPD analyses and morphological characteristics of cyst stages is also discussed.
引用
收藏
页码：19 / 26
页数：8
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