Virus interference with trans-plasma membrane activity in infected grapevine leaves

被引：3

作者：

Rinaldelli, Enrico ^{[1
]}

Luvisi, Andrea ^{[2
]}

Panattoni, Alessandra ^{[2
]}

机构：

[1] Univ Florence, Dept Agrifood Prod & Environm Sci, Sect Arboriculture, Lab Electrophysiol, I-50019 Florence, Italy

[2] Univ Pisa, Dept Agr Food & Environm, I-56124 Pisa, Italy

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2014年 / 36卷 / 12期

关键词：

t-PMEP; t-PMET; GLRaV-1; GLRaV-3; NADH; ELECTRON-TRANSPORT; ANTIVIRAL DRUGS; CELLS; PLANTS;

D O I：

10.1007/s11738-014-1695-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The trans-plasma membrane behavior in virus-infected grapevine leaves was investigated and the effects of six viruses included in European and Italian certification protocols of grapevine on trans-plasma membrane potential (t-PMEP) or electron transport (t-PMET) activity were evaluated. Electrophysiological tests were carried out on leaf samples of virus-infected Vitis vinifera cv. Sangiovese. Microelectrodes were placed in the central zone of the mesophyll for membrane potential measurement, while carbon fiber microelectrodes were used to estimate the membrane reductase activity of virus-infected resting cells. Viruses, the presence of which increased the NADH content, interfere differently with t-PMEP and t-PMET. Those that did not interfere negatively with membrane potential caused an increment in cell reductase activity, while virus-infected samples which showed a stressed status-as suggested by low energy availability and difficulty in the impalement procedure-were characterized by a lower t-PMET activity despite NADH content.

引用

页码：3345 / 3349

页数：5