The Trypanosoma cruzi genome project: Nuclear karyotype and gene mapping of clone CL Brener

被引：22
作者：
Santos, MRM
Cano, MI
Schijman, A
Lorenzi, H
Vazquez, M
Levin, MJ
Ramirez, JL
Brandao, A
Degrave, WM
deSilveira, JF
机构：
[1] UNIV FED SAO PAULO,UNIFESP EPM,BR-04023062 SAO PAULO,SP,BRAZIL
[2] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,INGEBI CONICET,INST INVEST INGN GENET & BIOL MOL,RA-1428 BUENOS AIRES,DF,ARGENTINA
[3] CENT UNIV VENEZUELA,CARACAS,VENEZUELA
[4] INST OSWALDO CRUZ,BR-21045900 RIO JANEIRO,RJ,BRAZIL
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1997年 / 92卷 / 06期
关键词：
Trypanosoma cruzi; clone CL Brener; genome project; molecular karyotype; chromosome mapping; genetic map;
D O I：
10.1590/S0074-02761997000600018
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
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收藏
页码：821 / 828
页数：8
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