Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y

被引：11

作者：

Kanosue, Y

Kojima, S

Ohkata, K

机构：

[1] Hiroshima Univ, Grad Sch Sci, Dept Chem, Higashihiroshima 7398526, Japan

[2] Hiroshima Univ, Ctr Quantum Life Sci, Higashihiroshima 7398526, Japan

来源：

JOURNAL OF PHYSICAL ORGANIC CHEMISTRY | 2004年 / 17卷 / 05期

关键词：

viscosity; induced fit process; carboxypeptidase-Y; dipeptides; hydrolysis;

D O I：

10.1002/poc.752

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The influence of solvent viscosity on the rate of enzymatic hydrolysis of a series of dipeptides (Z-Phe-Gly, Z-Phe-Sar, Z-Phe-Ala, Z-Phe-NMeAla, Z-Phe-Aib and Z-Phe-Pro) by carboxypeptidase Y was investigated. The effect of solvent viscosity on the enzymatic hydrolysis revealed that whereas all k(cat) values decreased with viscosity, those of the N-alkyl peptides decreased more than those of the N-H peptides. The kinetic behaviour implies the involvement of conformational changes of the enzyme in terms of the 'induced-fit' process. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：448 / 457

页数：10

共 47 条

[41] Ab initio evaluation of the potential surface for general base-catalyzed methanolysis of formamide:: A reference solution reaction for studies of serine proteases [J].