Influence of solvent viscosity on the rate of hydrolysis of dipeptides by carboxypeptidase Y

被引：11
作者：
Kanosue, Y
Kojima, S
Ohkata, K
机构：
[1] Hiroshima Univ, Grad Sch Sci, Dept Chem, Higashihiroshima 7398526, Japan
[2] Hiroshima Univ, Ctr Quantum Life Sci, Higashihiroshima 7398526, Japan
来源：
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY | 2004年 / 17卷 / 05期
关键词：
viscosity; induced fit process; carboxypeptidase-Y; dipeptides; hydrolysis;
D O I：
10.1002/poc.752
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The influence of solvent viscosity on the rate of enzymatic hydrolysis of a series of dipeptides (Z-Phe-Gly, Z-Phe-Sar, Z-Phe-Ala, Z-Phe-NMeAla, Z-Phe-Aib and Z-Phe-Pro) by carboxypeptidase Y was investigated. The effect of solvent viscosity on the enzymatic hydrolysis revealed that whereas all k(cat) values decreased with viscosity, those of the N-alkyl peptides decreased more than those of the N-H peptides. The kinetic behaviour implies the involvement of conformational changes of the enzyme in terms of the 'induced-fit' process. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码：448 / 457
页数：10
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