Plant-derived H7 VLP vaccine elicits protective immune response against H7N9 influenza virus in mice and ferrets

被引:50
|
作者
Pillet, S. [1 ,2 ]
Racine, T. [3 ]
Nfon, C. [4 ]
Di Lenardo, T. Z. [2 ]
Babiuk, S. [4 ,5 ]
Ward, B. J. [1 ]
Kobinger, G. P. [3 ,5 ,6 ]
Landry, N. [1 ]
机构
[1] Medicago Inc, Quebec City, PQ, Canada
[2] McGill Univ, Ctr Hlth, Res Inst, Montreal, PQ, Canada
[3] Publ Hlth Agcy Canada, Natl Microbiol Lab, Special Pathogens Program, Winnipeg, MB, Canada
[4] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada
[5] Univ Manitoba, Dept Immunol, Winnipeg, MB R3T 2N2, Canada
[6] Univ Penn, Dept Pathol & Lab Med, Sch Med, Philadelphia, PA USA
关键词
Plant-made vaccine; Virus-like particle; Influenza A; H7N9; Immunogenicity; Mice; Ferrets; NICOTIANA benthamiana; T-CELL RESPONSES; A VIRUS; PARTICLES; CD4(+); CHALLENGE; ADJUVANT; MODELS;
D O I
10.1016/j.vaccine.2015.09.065
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In March 2013, the Chinese Centre for Disease Control and Prevention confirmed the first reported case of human infection with an avian influenza A H7N9 virus. Infection with this virus often caused severe pneumonia and acute respiratory distress syndrome resulting in a case fatality rate >35%. The risk of pandemic highlighted, once again, the need for a more rapid and scalable vaccine response capability. Here, we describe the rapid (19 days) development of a plant-derived VLP vaccine based on the hemagglutinin sequence of influenza H7N9 A/Hangzhou/1/2013. The immunogenicity of the H7 VLP vaccine was assessed in mice and ferrets after one or two intramuscular dose(s) with and without adjuvant (alum or GLA-SE (TM)). In ferrets, we also measured H7-specific cell-mediated immunity. The mice and ferrets were then challenged with H7N9 A/Anhui/1/2013 influenza virus. A single immunization with the adjuvanted vaccine elicited a strong humoral response and protected mice against an otherwise lethal challenge. Two doses of unadjuvanted vaccine significantly increased humoral response and resulted in 100% protection with significant reduction of clinical signs leading to nearly asymptomatic infections. In ferrets, a single immunization with the alum-adjuvanted H7 VLP vaccine induced strong humoral and CMI responses with antigen-specific activation of CD3(+) T cells. Compared to animals injected with placebo, ferrets vaccinated with alum-adjuvanted vaccine displayed no weight loss during the challenge. Moreover, the vaccination significantly reduced the viral load in lungs and nasal washes 3 days after the infection. This candidate plant-made H7 vaccine therefore induced protective responses after either one adjuvanted or two unadjuvanted doses. Studies are currently ongoing to better characterize the immune response elicited by the plant-derived VLP vaccines. Regardless, these data are very promising for the rapid production of an immunogenic and protective vaccine against this potentially pandemic virus. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:6282 / 6289
页数:8
相关论文
共 50 条
  • [31] Adjuvanted recombinant hemagglutinin H7 vaccine to highly pathogenic influenza A(H7N9) elicits high and sustained antibody responses in healthy adults
    Christine M. Oshansky
    James King
    Di Lu
    James Zhou
    Corrina Pavetto
    Gary Horwith
    Karen Biscardi
    Bai Nguyen
    John J. Treanor
    Li-Mei Chen
    Brett Jepson
    Rick A. Bright
    Robert A. Johnson
    Vittoria Cioce
    Ruben O. Donis
    npj Vaccines, 6
  • [32] Immune Enhancement by the Tetrs-Peptide Hydrogel as a Promising Adjuvant for an H7N9 Vaccine against Highly Pathogenic H7N9 Virus
    Wu, Xiaoxin
    Tang, Songjia
    Wang, Zhehua
    Ma, Xiaoyun
    Zhang, Lingjian
    Zhang, Fen
    Xiao, Lanlan
    Zhao, Shuai
    Li, Qian
    Wang, Ying
    Wang, Qingjing
    Chen, Keda
    VACCINES, 2022, 10 (01)
  • [33] Antibodies to Antigenic Site A of Influenza H7 Hemagglutinin Provide Protection against H7N9 Challenge
    Schmeisser, Falko
    Vasudevan, Anupama
    Verma, Swati
    Wang, Wei
    Alvarado, Esmeralda
    Weiss, Carol
    Atukorale, Vajini
    Meseda, Clement
    Weir, Jerry P.
    PLOS ONE, 2015, 10 (01):
  • [34] A plant-derived VLP influenza vaccine elicits a balanced immune response even in very old mice with co-morbidities
    Hodgins, Breanna
    Pillet, Stephane
    Landry, Nathalie
    Ward, Brian James
    PLOS ONE, 2019, 14 (01):
  • [35] Protective efficacy of an inactivated chimeric H7/H5 avian influenza vaccine against highly pathogenic avian influenza H7N9 and clade 2.3.4.4 H5 viruses
    Peng, Cheng
    Hou, Guangyu
    Li, Jinping
    Wang, Suchun
    Wang, Yan
    Cheng, Shanju
    Yu, Xiaohui
    Jin, Jihui
    Jiang, Wenming
    VETERINARY MICROBIOLOGY, 2018, 223 : 21 - 26
  • [36] Suboptimal Humoral Immune Response against Influenza A(H7N9) Virus Is Related to Its Internal Genes
    Lee, Andrew C. Y.
    Zhu, Houshun
    Zhang, Anna J. X.
    Li, Can
    Wang, Pui
    Li, Chuangen
    Chen, Honglin
    Hung, Ivan F. N.
    To, Kelvin K. W.
    Yuen, Kwok-Yung
    CLINICAL AND VACCINE IMMUNOLOGY, 2015, 22 (12) : 1235 - 1243
  • [37] H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet
    Zhang, Qianyi
    Shi, Jianzhong
    Deng, Guohua
    Guo, Jing
    Zeng, Xianying
    He, Xijun
    Kong, Huihui
    Gu, Chunyang
    Li, Xuyong
    Liu, Jinxiong
    Wang, Guojun
    Chen, Yan
    Liu, Liling
    Liang, Libin
    Li, Yuanyuan
    Fan, Jun
    Wang, Jinliang
    Li, Wenhui
    Guan, Lizheng
    Li, Qimeng
    Yang, Huanliang
    Chen, Pucheng
    Jiang, Li
    Guan, Yuntao
    Xin, Xiaoguang
    Jiang, Yongping
    Tian, Guobin
    Wang, Xiurong
    Qiao, Chuanling
    Li, Chengjun
    Bu, Zhigao
    Chen, Hualan
    SCIENCE, 2013, 341 (6144) : 410 - 414
  • [38] H7N9 Live Attenuated Influenza Vaccine Is Highly Immunogenic, Prevents Virus Replication, and Protects Against Severe Bronchopneumonia in Ferrets
    de Jonge, Jorgen
    Isakova-Sivak, Irina
    van Dijken, Harry
    Spijkers, Sanne
    Mouthaan, Justin
    de Jong, Rineke
    Smolonogina, Tatiana
    Roholl, Paul
    Rudenko, Larisa
    MOLECULAR THERAPY, 2016, 24 (05) : 991 - 1002
  • [39] An H7N1 Influenza Virus Vaccine Induces Broadly Reactive Antibody Responses against H7N9 in Humans
    Krammer, Florian
    Jul-Larsen, Asne
    Margine, Irina
    Hirsh, Ariana
    Sjursen, Haakon
    Zambon, Maria
    Cox, Rebecca J.
    CLINICAL AND VACCINE IMMUNOLOGY, 2014, 21 (08) : 1153 - 1163
  • [40] H7N3 live attenuated influenza vaccine has a potential to protect against new H7N9 avian influenza virus
    Rudenko, Larisa
    Isakova-Sivak, Irina
    Donina, Svetlana
    VACCINE, 2013, 31 (42) : 4702 - 4705