Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract

被引：27

作者：

Sohal, Jasmeet Kaur ^{[1
]}

Saraf, Ashish ^{[1
]}

Shukla, Kamlesh Kumar ^{[2
]}

Shrivastava, Meghna ^{[1
]}

机构：

[1] MATS Univ, Fac Biol & Chem Sci, Raipur, Chhattisgarh, India

[2] Pt Ravishankar Shukla Univ, SoS Biotechnol, Raipur, Chhattisgarh, India

来源：

PLANT SCIENCE TODAY | 2019年 / 6卷 / 02期

关键词：

green synthesis; AgNPs; bioreduction; Aloe vera gel; characterization; antioxidant potential; METHANOLIC EXTRACTS; MECHANISMS; SYSTEMS; PHENOL; PLANT;

D O I：

10.14719/pst.2019.6.2.532

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Biosynthesis of nanoparticles by using plant extracts is presently under development. The study has been focused on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Aloe vera gel as well as to determine their antioxidant potential. UV-Vis spectrophotometeric analysis showed surface plasmonic resonance (SPR) band at 440 nm, which is specific for AgNPs. The Transmission Electron Microscopy (TEM) revealed that the synthesized AgNPs were spherical in shape with an average particle size of 66.6 nm. Fourier Transform Infrared Spectroscopic (FTIR) analysis of the aqueous extract before and after the synthesis of AgNPs revealed the presence of different functional groups related to phenolic and polyphenolic compounds such as tannins and flavonoids, and other metabolites like proteins, which may be responsible for the synthesis and stabilization of AgNPs. The antioxidant potential of the synthesized AGAgNPs was determined by using 2, 2 Diphenyl-1-Picryl Hydrazyl (DPPH) radical scavenging, metal chelating and reducing power assay. Antioxidant assessment showed enhanced dose dependent antioxidant potential of the synthesized AgNPs as compared to the crude extract, which can gain attention of the pharmaceutical industry for preparation of antioxidants of natural origin as the synthetic ones are suspected to be carcinogenic. Present study also supports the advantages of green method for the nanoparticles synthesis.

引用

页码：208 / 217

页数：10

共 41 条

[1] Role of reactive oxygen species in the pathophysiology of human reproduction [J].

Agarwal, A ;

Saleh, RA ;

Bedaiwy, MA .

FERTILITY AND STERILITY, 2003, 79 (04) :829-843

[2] Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum [J].

Ahmad, A ;

Mukherjee, P ;

Senapati, S ;

Mandal, D ;

Khan, MI ;

Kumar, R ;

Sastry, M .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2003, 28 (04) :313-318

[3] Rapid synthesis of silver nanoparticles using dried medicinal plant of basil [J].

Ahmad, Naheed ;

Sharma, Seema ;

Alam, Md. K. ;

Singh, V. N. ;

Shamsi, S. F. ;

Mehta, B. R. ;

Fatma, Anjum .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2010, 81 (01) :81-86

[4]

Anilakumar KR, 2010, INDIAN J EXP BIOL, V48, P837

[5] Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity [J].

Bhakya, S. ;

Muthukrishnan, S. ;

Sukumaran, M. ;

Muthukumar, M. .

APPLIED NANOSCIENCE, 2016, 6 (05) :755-766

[6]

Bibi G, 2012, J PHARM HARB FORM, V2, P33

[7] ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].

BLOIS, MS .

NATURE, 1958, 181 (4617) :1199-1200

[8]

Bruchlausen F.V, 1979, N-S ARCH PHARMACOL, V308, P27

[9] Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1 [J].

Chung, YC ;

Chang, CT ;

Chao, WW ;

Lin, CF ;

Chou, ST .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (08) :2454-2458

[10] ROLE OF FERRITIN AS A LIPID OXIDATION CATALYST IN MUSCLE FOOD [J].

DECKER, EA ;

WELCH, B .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (03) :674-677

← 1 2 3 4 5 →