Structural characterization and DPPH• radical scavenging activity of a polysaccharide from Guara fruits

被引：83

作者：

Hua, Dehong ^{[1
]}

Zhang, Dezhi ^{[1
]}

Huang, Bing ^{[1
]}

Yi, Pan ^{[1
]}

Yan, Chunyan ^{[1
]}

机构：

[1] Guangdong Pharmaceut Univ, Coll Pharm, Guangzhou 510006, Guangdong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 103卷

基金：

中国国家自然科学基金;

关键词：

Psidium gua[!text type='java']java[!/text] Linn; Polysaccharides; Structure; DPPH center dot radical scavenging; PSIDIUM-GUA[!text type='JAVA']JAVA[!/text; ANTIOXIDANT ACTIVITY; IN-VITRO; ARABINOGALACTAN; PURIFICATION; PULP;

D O I：

10.1016/j.carbpol.2013.12.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The crude polysaccharides were extracted from fruits of Psidium guajava Linn. by hot water. After removal of proteins, isolation and purification by DEAE-52 Cellulose chromatography and Sephadex G-75 gel filtration, a polysaccharide (GP70-2) was obtained and structurally characterized. GP70-2 has a relative molecular weight of 74 kDa and was composed of D-galactose and L-arabinose in the ratio of 1:1, with a specific optical rotation of [a](D)(25) = +101 degrees. Structural characterization of this novel polysaccharide was carried out using infrared spectroscopy, methylation analyses, and NMR studies (H-1, C-13, H-1-H-1-COSY, HMQC, and HMBC). Based on the above data, the following structure was assigned to the repeated core unit of GP70-2: -> 3)-alpha-L- Araf-(1 -> 3)-beta-D-Galn-(1 -> 3)-alpha-L- Araf-(1 -> 3) - alpha-L- Araf-(1 ->(6 <- 1)- beta-D-Galp-(6 -> 1) -beta-d-Galp This polysaccharide showed a concentration dependent DPPH center dot radical scavenging activity. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：143 / 147

页数：5

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