Dopamine transporter mRNA in autopsy studies of chronic cocaine users

被引：14
作者：
Chen, L
Segal, DM
Moraes, CT
Mash, DC
机构：
[1] Univ Miami, Sch Med, Dept Neurol, Miami, FL 33136 USA
[2] Univ Miami, Sch Med, Dept Mol & Cellular Pharmacol, Miami, FL 33136 USA
来源：
MOLECULAR BRAIN RESEARCH | 1999年 / 73卷 / 1-2期
关键词：
cocaine; reverse transcription; polymerase chain reaction; mRNA; human brain; cocaine delirium;
D O I：
10.1016/S0169-328X(99)00233-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The effects of chronic cocaine abuse on transcriptional regulation of human dopamine transporter (DAT) mRNA in midbrain dopaminergic neurons was assessed by reverse transcription-polymerase chain reaction (RT-PCR). DAT/cyclophilin mRNA ratios in the substantia nigra (SN) were unchanged in cocaine overdose (CO) victims as compared to age-matched and drug free control (CTRL) subjects. In contrast, DAT mRNA levels were decreased significantly in agitated cocaine delirium victims (67%, p < 0.05). These findings demonstrate the lack of a regulatory influence by cocaine on the steady state content of DAT mRNA in cocaine abusers. In contrast, DAT gene expression was altered significantly in cocaine abusers at risk for agitated delirium. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：181 / 185
页数：5
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