Inhibition of lung tumor growth in nude mice by siRNACD31 targeting PECAM-1

被引：14

作者：

Ouyang, Jin-Sheng ^{[1
]}

Li, Yu-Ping ^{[1
]}

Chen, Cheng-Shui ^{[1
]}

Chen, Jun-Jie ^{[1
]}

Chen, Tong-Ke ^{[1
]}

Cai, Chang ^{[1
]}

Yang, Li ^{[1
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Resp Med, Wenzhou 32500, Zhejiang, Peoples R China

来源：

ONCOLOGY LETTERS | 2014年 / 8卷 / 01期

关键词：

small interfering RNA; platelet endothelial adhesion molecule; vascular endothelial growth factor; carcinoma; tumor microenvironment; NONSMALL CELL LUNG; VASCULAR ENDOTHELIAL-CELLS; ADHESION MOLECULE-1; SIGNALING PATHWAY; IN-VITRO; CANCER; PLATELET; ANGIOGENESIS; CHEMOTHERAPY; BEVACIZUMAB;

D O I：

10.3892/ol.2014.2091

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease-causing genes. In the present study, the use of 2'-O-methyl-modified siRNA-cluster of differentiation 31 (siRNA(CD31)), with cationic liposome RNA interference (RNAi)-mate as a carrier, effectively silenced the platelet endothelial cell molecule 1 (PECAM-1) gene of murine hemangioendothelioma cells in vitro. In vivo, 2'-O-methyl-modified siRNA(CD31) carried by RNAi-mate was successfully delivered, targeting the PECAM-1 gene in the vasculature of nude mouse lung carcinoma xenografts. The growth of the lung carcinoma xenografts was inhibited by the 2'-O-methyl-modified siRNA(CD31) and RNAi-mate complexes, and the expression of the PECAM-1 protein was downregulated, with a simultaneous decrease in vascular endothelial growth factor (VEGF) protein in the lung carcinoma xenografts. 2'-O-methyl-modified siRNA(CD31)-RNAi-mate complexes may provide a potential therapeutic strategy in lung carcinoma treatment. The effect of PECAM-1 on VEGF expression may possibly be attributed to the function of PECAM-1 signal transduction.

引用

页码：33 / 40

页数：8

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