Structural Study of Silk-like Peptides Modified by the Addition of the Cell Adhesive Sequence, RGD, Using 13C CP/MAS NMR

被引：2

作者：

Tanaka, Chikako ^{[1
,2
]}

Asano, Atsushi ^{[2
]}

Kurotsu, Takuzo ^{[2
]}

Asakura, Tetsuo ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[2] Natl Def Acad, Dept Appl Chem, Yokosuka, Kanagawa 2398686, Japan

来源：

POLYMER JOURNAL | 2009年 / 41卷 / 01期

关键词：

Silk-like Peptide; Anaphe Silk Fibroin; Cell Adhesive Sequence RGD; C-13; CP/MAS; NMR; X-RAY-DIFFRACTION; SOLID-STATE; FIBROIN; FIBRONECTIN; SPECTROSCOPY; MODEL;

D O I：

10.1295/polymj.PJ2008218

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We synthesized the 13C-labeled peptides with the sequences (AAG)n1 or (AG)n2 in both N- and C-terminal parts and the cell-adhesive sequence, ASTGRGDSPAAS in the central part in order to obtain basic information to design the cell-adhesive silk-like proteins. The 13C CP/MAS NMR was used to characterize these structures in the solid states. One of the possible structures was shown. © 2009 The Society of Polymer Science.

引用

页码：18 / 19

页数：2

共 7 条

[1] CONFORMATION CHARACTERIZATION OF BOMBYX-MORI SILK FIBROIN IN THE SOLID-STATE BY HIGH-FREQUENCY C-13 CROSS POLARIZATION MAGIC ANGLE SPINNING NMR, X-RAY-DIFFRACTION, AND INFRARED-SPECTROSCOPY
ASAKURA, T
KUZUHARA, A
TABETA, R
SAITO, H
[J]. MACROMOLECULES, 1985, 18 (10) : 1841 - 1845
[2] Heterogeneous structure of silk fibers from Bombyx mori resolved by 13C solid-state NMR spectroscopy
Asakura, T
Yao, JM
Yamane, T
Umemura, K
Ulrich, AS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (30) : 8794 - 8795
[3] Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin
Asakura, T
Tanaka, C
Yang, MY
Yao, JM
Kurokawa, M
[J]. BIOMATERIALS, 2004, 25 (04) : 617 - 624
[4] A repeated β-turn structure in poly(Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance
Asakura, T
Ashida, J
Yamane, T
Kameda, T
Nakazawa, Y
Ohgo, K
Komatsu, K
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 306 (02) : 291 - 305
[5] THE 3-DIMENSIONAL STRUCTURE OF THE 10TH TYPE-III MODULE OF FIBRONECTIN - AN INSIGHT INTO RGD-MEDIATED INTERACTIONS
MAIN, AL
HARVEY, TS
BARON, M
BOYD, J
CAMPBELL, ID
[J]. CELL, 1992, 71 (04) : 671 - 678
[6] Structural analyses of Anaphe silk fibroin and several model peptides using 13C NMR and X-ray diffraction methods
Tanaka, Chikako
Takahashi, Rui
Asano, Atsushi
Kurotsu, Takuzo
Akai, Hirornu
Sato, Kazuhiko
Knight, David P.
Asakura, Tetsuo
[J]. MACROMOLECULES, 2008, 41 (03) : 796 - 803
[7] Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin
Yang, Mingying
Tanaka, Chikako
Yamauchi, Kazuo
Ohgo, Kosuke
Kurokawa, Masato
Asakura, Tetsuo
[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 84A (02) : 353 - 363

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