Structural Study of Silk-like Peptides Modified by the Addition of the Cell Adhesive Sequence, RGD, Using 13C CP/MAS NMR

被引：2

作者：

Tanaka, Chikako ^{[1
,2
]}

Asano, Atsushi ^{[2
]}

Kurotsu, Takuzo ^{[2
]}

Asakura, Tetsuo ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[2] Natl Def Acad, Dept Appl Chem, Yokosuka, Kanagawa 2398686, Japan

来源：

POLYMER JOURNAL | 2009年 / 41卷 / 01期

关键词：

Silk-like Peptide; Anaphe Silk Fibroin; Cell Adhesive Sequence RGD; C-13; CP/MAS; NMR; X-RAY-DIFFRACTION; SOLID-STATE; FIBROIN; FIBRONECTIN; SPECTROSCOPY; MODEL;

D O I：

10.1295/polymj.PJ2008218

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We synthesized the 13C-labeled peptides with the sequences (AAG)n1 or (AG)n2 in both N- and C-terminal parts and the cell-adhesive sequence, ASTGRGDSPAAS in the central part in order to obtain basic information to design the cell-adhesive silk-like proteins. The 13C CP/MAS NMR was used to characterize these structures in the solid states. One of the possible structures was shown. © 2009 The Society of Polymer Science.

引用

页码：18 / 19

页数：2

共 7 条

[1] CONFORMATION CHARACTERIZATION OF BOMBYX-MORI SILK FIBROIN IN THE SOLID-STATE BY HIGH-FREQUENCY C-13 CROSS POLARIZATION MAGIC ANGLE SPINNING NMR, X-RAY-DIFFRACTION, AND INFRARED-SPECTROSCOPY [J].

ASAKURA, T ;

KUZUHARA, A ;

TABETA, R ;

SAITO, H .

MACROMOLECULES, 1985, 18 (10) :1841-1845

[2] Heterogeneous structure of silk fibers from Bombyx mori resolved by 13C solid-state NMR spectroscopy [J].

Asakura, T ;

Yao, JM ;

Yamane, T ;

Umemura, K ;

Ulrich, AS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (30) :8794-8795

[3] Production and characterization of a silk-like hybrid protein, based on the polyalanine region of Samia cynthia ricini silk fibroin and a cell adhesive region derived from fibronectin [J].

Asakura, T ;

Tanaka, C ;

Yang, MY ;

Yao, JM ;

Kurokawa, M .

BIOMATERIALS, 2004, 25 (04) :617-624

[4] A repeated β-turn structure in poly(Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance [J].

Asakura, T ;

Ashida, J ;

Yamane, T ;

Kameda, T ;

Nakazawa, Y ;

Ohgo, K ;

Komatsu, K .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 306 (02) :291-305

[5] THE 3-DIMENSIONAL STRUCTURE OF THE 10TH TYPE-III MODULE OF FIBRONECTIN - AN INSIGHT INTO RGD-MEDIATED INTERACTIONS [J].

MAIN, AL ;

HARVEY, TS ;

BARON, M ;

BOYD, J ;

CAMPBELL, ID .

CELL, 1992, 71 (04) :671-678

[6] Structural analyses of Anaphe silk fibroin and several model peptides using 13C NMR and X-ray diffraction methods [J].

Tanaka, Chikako ;

Takahashi, Rui ;

Asano, Atsushi ;

Kurotsu, Takuzo ;

Akai, Hirornu ;

Sato, Kazuhiko ;

Knight, David P. ;

Asakura, Tetsuo .

MACROMOLECULES, 2008, 41 (03) :796-803

[7] Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin [J].

Yang, Mingying ;

Tanaka, Chikako ;

Yamauchi, Kazuo ;

Ohgo, Kosuke ;

Kurokawa, Masato ;

Asakura, Tetsuo .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 84A (02) :353-363

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