Ratiometric Fluorescence Capillary Sensor-Integrated Molecular Imprinting for Simultaneous Detection of Two Biological Indicators of Parkinson?s Disease

This work proposed ratiometric fluorescence capillary sensing system integrated molecular imprinting with highly sensitive and selective detection for two biological indicators of Parkinson's disease (homovanillic acid (HVA) and Al3+). In this research, the silicon carbon quantum dot and the near-infrared CdTe quantum dot as luminescence sources were doped to an imprinted layer, which was attached to the inner surface wall of an amino-functionalized capillary. The fluorescence emissions of the ratiometric fluorescence capillary-imprinted sensor at 434 and 707 nm were quenched by HVA, and only the fluorescence emission at 434 nm was quenched by Al3+. Ratiometric fluorescence capillary sensing system-integrated molecular imprinting was used to detect simultaneously HVA and Al3+ with linearity over 1.0 x 10-9-2.5 x 10-7 and 1.0 x 10-9-1.1 x 10-7 M, respectively. The sensor showcased detection limitations of 8.7 x 10-10 and 9.8 x 10-10 M, indicating that the ratiometric fluorescence capillary sensing system-integrated molecular imprinting had great potential application for detecting HVA and Al3+ in serum and urine samples. The ratiometric fluorescence capillary sensing system-integrated molecular imprinting achieved highly sensitive and selective detection of HVA and Al3+ with a microvolume test dosage of 18 mu L, which provided a new way for early diagnosis and disease monitoring of Parkinson's disease.