Chromosomal assignment of AFLP markers in upland cotton (Gossypium hirsutum L.)

In this research, we used two sets of cotton aneuploid (G. hirsutum x G. tomentosum and G. hirsutum x G. barbadense) plants to locate AFLP markers to chromosomes using deletion analysis method. Thirty-eight primer combinations were used to generate 608 polymorphic AFLP markers. A total of 98 AFLP markers were assigned to 22 different cotton chromosomes or chromosome arms. Of those assigned markers, 63.3% were assigned to A genome and 36.7% were assigned to D genome. A low rate (14.3%) of common markers were found between those assigned AFLP markers with the AFLP markers from an intraspecific cross population developed previous in our lab. Based on the 16 common markers, we were able to associate the 13 linkage groups previously identified in our lab to eight chromosomes. Further research will be carried out by using SSR markers with known location to associate unassigned linkage groups to chromosomes.