Structure, measurement, and secretion of human glucagon-like peptide-2

被引：192
作者：
Hartmann, B
Johnsen, AH
Orskov, C
Adelhorst, K
Thim, L
Holst, JJ
机构：
[1] Univ Copenhagen, Panum Inst, Dept Med Physiol, DK-2200 Copenhagen N, Denmark
[2] Univ Copenhagen, Panum Inst, Dept Med Anat, DK-2200 Copenhagen, Denmark
[3] Rigshosp, Dept Clin Biochem, DK-2100 Copenhagen 0, Denmark
[4] Novo Nordisk AS, DK-2880 Bagsvaerd, Denmark
来源：
PEPTIDES | 2000年 / 21卷 / 01期
关键词：
proglucagon; radioimmunoassay; GLP-2; GLP-1; DPP-IV;
D O I：
10.1016/S0196-9781(99)00176-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals. (C) 2000 Elsevier Science Inc. All rights reserved.
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页码：73 / 80
页数：8
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