Fluorescein-based pl markers for capillary isoelectric focusing with laser-induced fluorescence detection

被引：0
作者：
Slais, K
Horká, M
Novácková, J
Friedl, Z
机构：
[1] Acad Sci Czech Republ, Inst Analyt Chem, CZ-61142 Brno, Czech Republic
[2] Brno Univ Technol, Fac Chem, Brno, Czech Republic
来源：
ELECTROPHORESIS | 2002年 / 23卷 / 11期
关键词：
capillary isoelectric focusing; fluorescence detection; pl standards; protein separation;
D O I：
10.1002/1522-2683(200206)23:11<1682::AID-ELPS1682>3.0.CO;2-Q
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We prepared a series of low-molecular-mass fluorescent ampholytes with narrow p/ range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent p/ markers, were examined. The p/ values of new fluorescein-based p/ markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass p/ markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
引用
收藏
页码：1682 / 1688
页数：7
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