A method for bacteriocin quantification

被引：107
作者：
Cabo, ML [1 ]
Murado, MA [1 ]
González, MP [1 ]
Pastoriza, L [1 ]
机构：
[1] CSIC, Inst Invest Marinas, Vigo 36208, Pontevedra, Spain
来源：
JOURNAL OF APPLIED MICROBIOLOGY | 1999年 / 87卷 / 06期
关键词：
D O I：
10.1046/j.1365-2672.1999.00942.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared. As an alternative designed to overcome the problems which characterize the response of micro-organisms that are sensitive to bacteriocins, an operative procedure in a liquid medium and a modified re-parameterized logistic equation is proposed. When applied to the inhibition of Leuconostoc mesenteroides by nisin, the model allows an optimal experimental procedure to be defined.
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收藏
页码：907 / 914
页数：8
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